Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1.1(CAG-Pycard/mCitrine*,-CD2*)Dtg
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Douglas T Golenbock
MGI ID:	MGI:5903223
Synonyms:	ASC-citrine
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1.1(CAG-Pycard/mCitrine*,-CD2*)Dtg page

Germline Transmission:	Earliest citation of germline transmission: J:238511
Parent Cell Line:	Bruce 4 (ES Cell)
Strain of Origin:	B6.Cg-Thy1a

Allele Type:    Targeted (Reporter) Mutation:    Insertion   Mutation details: The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a loxP-flanked STOP cassette, the mASC-citrine-HA fusion protein (described below), an FRT site, an iIRES, a portion from the human CD2 antigen locus encoding a truncated signaling-deficient protein (hCD2*), a second FRT site and a BGH polyA signal. The targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The mASC-citrine-HA fusion protein cDNA sequence encodes the mouse PYD and CARD domain containing coding region fused to a Citrine variant of yellow fluorescent protein, all followed by a hemagglutinin tag sequence. The Citrine used here is a yellow fluorescent protein variant with the K69A and A206K mutations (for improved brightness and monomeric stability) - encoding a ~5.7 pKa monomer with ~488nm excitation and ~529nm emmission spectra. Cre-mediated recombination removed the upstream loxP-flanked STOP cassette. (J:238511)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:238511 Tzeng TC, et al., A Fluorescent Reporter Mouse for Inflammasome Assembly Demonstrates an Important Role for Cell-Bound and Free ASC Specks during In Vivo Infection. Cell Rep. 2016 Jul 12;16(2):571-82
All:	14 reference(s)