Probe:	Dach1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present		5A
TS13: lateral plate mesoderm	Present		5A
TS13: trunk mesenchyme	Present		5A
TS13: neural fold	Weak		5A
TS13: neural plate	Weak	Regionally restricted	5A	Expression is absent in the anterior neural plate.
TS13: neural groove	Absent		5A

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Regionally restricted	5B	Expression is dorsal.
TS15: future forebrain	Present		5B
TS15: future hindbrain	Present		5B
TS15: optic vesicle	Present		5B
TS15: otocyst	Present		5B
TS15: somite	Present	Regionally restricted	5B	Expression is posterior in the caudal somites.
TS15: trunk mesenchyme	Present	Regionally restricted	5B	Expression is in the lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	5C	Expression is dorsal.
TS17: dorsal root ganglion	Present		5C
TS17: forelimb bud mesenchyme	Present		5C
TS17: hindbrain	Present		5C
TS17: hindlimb bud mesenchyme	Present		5C
TS17: midbrain	Present		5C
TS17: optic cup	Present		5C
TS17: telencephalon	Present		5C
TS17: trigeminal ganglion	Present		5C
TS17: outer ear	Present	Regionally restricted	5C	Expression is in the pinna.
TS17: endolymphatic appendage	Present	Regionally restricted	5C	Expression is in the endolymphatic duct.
TS17: branchial arch mesenchyme	Present	Regionally restricted	5C	Expression is at the boundaries.
TS17: trunk mesenchyme	Present	Regionally restricted	5C	Expression is in myogenic cells migrating into the inter-limb flank.
TS17: myotome	Present	Regionally restricted	5C	Expression is in the posterior half.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	5D	Expression is dorsal.
TS22: genital tubercle	Present		5D
TS22: hindbrain	Present		5D
TS22: midbrain	Present		5D
TS22: pinna	Present		5D
TS22: retina	Present		5D
TS22: telencephalon	Present		5D
TS22: limb	Present	Regionally restricted	5D	Expression is in the distal tips of the limb buds.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	5E	Expression is dorsal.
TS17: dorsal root ganglion	Present		5E
TS17: spinal ganglion	Present		5E
TS17: myotome	Present	Regionally restricted	5E	Expression is in the posterior half.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	5F	Expression is dorsal.
TS20: dorsal root ganglion	Present		5F
TS20: myotome	Present		5F

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	5G	Expression is in the dorsal area and is weak in the ventral neural tube.
TS17: dorsal root ganglion	Present		5G
TS17: forelimb bud mesenchyme	Present		5G
TS17: myotome	Present		5G

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	5H	Expression is in the dorsal area and is weak in the ventral neural tube.
TS17: dorsal root ganglion	Present		5H
TS17: hindlimb bud mesenchyme	Present		5H
TS17: myotome	Present		5H
TS17: trunk mesenchyme	Present	Regionally restricted	5H	Expression is in myogenic cells.

Structure	Level	Pattern	Image	Note
TS24: retina inner nuclear layer	Strong		6A
TS24: retina layer	Strong		6A
TS24: retina nerve fiber layer	Strong		6A
TS24: retina outer nuclear layer	Strong		6A
TS24: lens anterior epithelium	Weak		6B , 6A
TS24: lens equatorial epithelium	Weak		6B , 6A

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	6C	Expression is in areas surrounding the optic recess.
TS20: cerebellum primordium	Present		6C
TS20: cerebral cortex	Present		6C
TS20: ganglionic eminence	Present		6C
TS20: hypothalamus	Present		6C
TS20: medulla oblongata	Present		6C
TS20: midbrain roof plate	Present		6C
TS20: pons	Present		6C
TS20: thalamus	Present		6C
TS20: non-neural ectoderm	Absent		6C

Structure	Level	Pattern	Image	Note
TS20: hair follicle	Present	Regionally restricted	6D	Expression is in the epithelium surrounding the hair shaft.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	7A	Expression is in a stripe along the proximal-distal axis of the limb, with highest expression at the anterior and posterior margins of the limb bud.
TS17: forelimb bud ectoderm	Absent		7A

Structure	Level	Pattern	Image	Note
TS21: hand	Present		7B
TS21: forelimb digit	Strong	Regionally restricted	7B	Expression is at the distal tips.

Structure	Level	Pattern	Image	Note
TS22: forelimb phalanx cartilage condensation	Present	Regionally restricted	7C	Expression is in the perichondrium.
TS22: hand	Present		7C

Structure	Level	Pattern	Image	Note
TS17: retina	Present

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present