Antibody:	Sodium/Calcium Exchanger Monoclonal Antibody (C2C12)
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Punctate	3A	Present in the neural ectoderm extending to the lateral ectoderm.
TS10: embryo mesoderm	Present	Punctate	3A	Expressed in the lateral mesoderm on the right side of the embryo.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Punctate	3B	Present in the neural ectoderm extending to the lateral ectoderm. Primarily punctate and generally distributed throughout the ectoderm, along cell membrane boundaries, especially at the interface of the ectoderm and mesoderm.
TS10: embryo mesoderm	Present	Punctate	3B	Expressed in the lateral mesoderm on the right side of the embryo. Primarily punctate and generally distributed throughout the ectoderm, along cell membrane boundaries, especially at the interface of the ectoderm and mesoderm.

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present	Punctate	4A	Expressed in cells at the mesoderm-endoderm interface in the anterior heart-forming regions. Primarily in the endoderm and at the basal aspect of the epithelial cardiac mesoderm.

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present	Punctate	4B	Expressed in the endoderm, underlying the cardiac mesoderm.

Structure	Level	Pattern	Image	Note
TS12: outflow tract	Present	Punctate	5A	Expressed in a sparse, punctate pattern.

Structure	Level	Pattern	Image	Note
TS12: outflow tract	Present	Punctate	5B	Expressed in a sparse, punctate pattern.
TS12: primitive ventricle cardiac muscle	Present		5B

Structure	Level	Pattern	Image	Note
TS12: primitive ventricle cardiac muscle	Present	Non-Uniform	5C	The left side of the heart tube shows more signal in the cells than the right side.

Structure	Level	Pattern	Image	Note
TS12: primitive ventricle cardiac muscle	Present	Non-Uniform	5D	Expression in the outer and inner curvature of the heart than in other regions of the myocardium.

Structure	Level	Pattern	Image	Note
TS12: sinus venosus	Trace		5E	Little signal is detectable.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present	Widespread	6A	Expressed more uniformly than in day-8 embryos. Most cells of the myocardium, primarily within the ventricular regions.
TS15: primitive ventricle cardiac muscle	Present	Widespread	6A	Expressed in most cells.

Structure	Level	Pattern	Image	Note
TS15: heart	Strong	Regionally restricted	6B	High expression on the ventral aspect of the heart tube and also in association with the dorsal mesocardium.
TS15: cardiac muscle tissue	Present		6D , 6C , 6B
TS15: endocardial lining	Present		6D , 6C , 6B

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present	Widespread	6F , 6E	A relatively high expression throughout the myocardium and endocardium.
TS15: atrium cardiac muscle	Present	Widespread	6F , 6E	A relatively high expression.
TS15: atrium endocardial lining	Present	Widespread	6F , 6E	A relatively high expression.
TS15: primitive ventricle cardiac muscle	Present	Widespread	6F , 6E	A relatively high expression.
TS15: primitive ventricle endocardial lining	Present	Widespread	6F , 6E	A relatively high expression.

Structure	Level	Pattern	Image	Note
TS15: sinus venosus	Weak		6G

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Present		6H
TS15: primitive ventricle endocardial lining	Present		6H