About   Help   FAQ
Rasal2Tn(pb-Act-RFP)1Xwu
Transposon induced Allele Detail
Summary
Symbol: Rasal2Tn(pb-Act-RFP)1Xwu
Name: RAS protein activator like 2; transposon insertion 1, Xiaohui Wu
MGI ID: MGI:5897630
Gene: Rasal2  Location: Chr1:156962759-157240170 bp, - strand  Genetic Position: Chr1, 67.97 cM
Alliance: Rasal2Tn(pb-Act-RFP)1Xwu page
Mutation
origin
Strain of Origin:  FVB/NJ
Mutation
description
Allele Type:    Transposon induced (Hypomorph, Reporter)
Mutation:    Transposon insertion
 
Mutation detailsA piggyBac (PB) transposon element derived from cabbage looper moth Trichoplusia ni is introduced to intron 3 of the gene. Its components were introduced by coinjection of mouse oocytes with circular piggyBac donor and helper plasmids. The donor plasmid contained the piggyBac transposon element in which the transposase sequence was replaced by a beta actin (Actb) promoter-driven red flourescent protein (RFP) reporter gene flanked by piggyBac-specific inverted terminal repeat sequences. The helper plasmid employs the Actb promoter to drive expression of a piggyBac transposase gene lacking the terminal inverted repeat sequences required for its transposition. The insertion contains the RFP expression unit flanked by transposon (donor) and transposase (helper) sequences. RFP is flanked by transposon donor and transposase helper sequences in the final construct. Expression of Rasal2 is decreased by more than 90% in some tissues. (J:101977)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Rasal2 Mutation:  59 strains or lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory