Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Thy1-jRGECO1a)GP8.31Dkim
Name:	transgene insertion GP8.31, Douglas Kim
MGI ID:	MGI:5896540
Synonyms:	GP8.31
Transgene:	Tg(Thy1-jRGECO1a)GP8.31Dkim  Location: unknown
Alliance:	Tg(Thy1-jRGECO1a)GP8.31Dkim page

Strain of Origin:

C57BL/6J

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The mouse Thy1 promoter drives expression of this transgene that includes jRGECO1a, nuclear export signal (NES) from protein kinase inhibitor alpha (PKIalpha), woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation (polyA) sequence. jRGECO1a is a modified form of R-GECO. R-GECO is derived from mApple (a red fluorescent monomer which is substantially brighter than mCherry and displays complex photoswitching behavior) fused to calmodulin and the calmodulin binding region of chicken myosin light chain kinase (M13). The calmodulin region is modified with Q306D and M339F mutations. The M339F mutation accelerates kinetics, but does not affect response amplitude. For cortical imaging, founder line GP8.20 shows denser labeling (~60% of cells) and founder line GP8.31 shows sparser labeling (~30% of all cells). Both GP8.20 and GP8.31 both provide good signal levels across most of the cortex. (J:268005)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:268005 Dana H, et al., Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population imaging in vivo. PLoS One. 2018;13(10):e0205444
All:	2 reference(s)