Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:214712
Le Bras B, et al., In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014 Jul;219(4):1433-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5828263
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Gene symbol:
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Nfasc
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Gene name:
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neurofascin
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Antibody:
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NFC1 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
6a2:
embryonic day 9.0
(more )
Specimen
6a2:
(close )
Genetic Background:
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CRL:OF1
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Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
2% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
6b2:
embryonic day 9.25
(more )
Note: Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
Specimen
6b2:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 9.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube lateral wall |
Present |
Regionally restricted |
6b2
,
6b3
|
Expression is in a few motor neurons and along their axons extending out of the spinal cord.
|
Specimen
6c2:
embryonic day 10.5
(more )
Note: Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
Specimen
6c2:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube lateral wall |
Present |
Regionally restricted |
6c2
,
6c3
|
Expression is in motor neurons and along their axons extending out of the spinal cord.
|
Specimen
6d2:
embryonic day 11.5
(more )
Note: Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
Specimen
6d2:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
6d2
,
6d3
|
Expression is in the axons of motor neurons with a distal to proximal restriction.
|
Specimen
6e2:
embryonic day 13.5
(more )
Note: Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
Specimen
6e2:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Nfasc; green - Ank3 (assay MGI:5828213).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: basal columns |
Present |
Regionally restricted |
6e2
,
6e3
|
Expression is in the proximal axonal segments of motor neurons in the spinal cord.
|
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