Probe:	Chrna2 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: basal forebrain	Present	Regionally restricted	1A	Expression in basal magnocellular complex (BMC).`

Structure	Level	Pattern	Image	Note
TS22: basal forebrain	Present	Regionally restricted	1B	Expression in basal magnocellular complex (BMC).
TS22: pallidum	Present	Regionally restricted	1B	Expression in a few scattered cells in the ventral pallidum (VP).
TS22: globus pallidus	Absent		1B

Structure	Level	Pattern	Image	Note
TS22: basal forebrain	Present	Regionally restricted	1C	Expression in basal magnocellular complex (BMC).
TS22: amygdala	Present	Regionally restricted	1C	No expression in central amygdala (Ce).

Structure	Level	Pattern	Image	Note
TS22: pallidum	Present	Regionally restricted	1D	Expression in a few scattered cells in the ventral pallidum (VP).
TS22: globus pallidus	Absent		1D

Structure	Level	Pattern	Image	Note
TS22: pallidum	Present	Regionally restricted	1E	Expression in bed nucleus of stria terminalis (BST).

Structure	Level	Pattern	Image	Note
TS22: pallidum	Present	Regionally restricted	1F	Expression in bed nucleus of stria terminalis (BST).

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present	Regionally restricted	1G	Expression in ventromedial extended amygdala (EA). No expression in central amygdala (Ce).
TS22: globus pallidus	Absent		1G

Structure	Level	Pattern	Image	Note
TS22: pallidum	Present	Regionally restricted	1H	Expression in bed nucleus of stria terminalis (BST).
TS22: globus pallidus	Absent		1H

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present	Regionally restricted	1I	Expression in ventromedial extended amygdala (EA).
TS22: pallidum	Present	Regionally restricted	1I	Expression in bed nucleus of stria terminalis (BST).
TS22: globus pallidus	Absent		1I

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present	Regionally restricted	1J	Expression in cells distributed in area corresponding to the ventromedial extended amygdala (EA). No expression in striatal amygdala.
TS22: pallidum	Present	Regionally restricted	1J	Expression in a few scattered cells in the ventral pallidum (VP).
TS22: globus pallidus	Absent		1J
TS22: piriform cortex	Absent		1J

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present	Regionally restricted	1K	Expression in cells distributed in area corresponding to the ventromedial extended amygdala (EA).
TS22: globus pallidus	Absent		1K
TS22: piriform cortex	Absent		1K

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present	Regionally restricted	1L	No expression in basomedial amygdalar nucleus (BM) and basolateral amydalar nucleus (BL). No expression in cortical amygdala (ACx).
TS22: globus pallidus	Absent		1L
TS22: piriform cortex	Absent		1L

Structure	Level	Pattern	Image	Note
TS22: basal forebrain	Present	Regionally restricted	2A	Expression in basal magnocellular complex (BMC).
TS22: amygdala	Present	Regionally restricted	2A	Intense expression in cells distributed in area corresponding to the ventromedial extended amygdala (EA).

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present	Regionally restricted	2D	No expression in amygdalar areas such as medial amygdala, posteroventral part (MePV).

Structure	Level	Pattern	Image	Note
TS22: basal forebrain	Present		2G	Expression in basal magnocellular complex (BMC).
TS22: amygdala	Present		2G	No expression in amygdalar areas such as medial amygdala, posteroventral part (MePV).

Structure	Level	Pattern	Image	Note
TS24: basal forebrain	Present	Regionally restricted	3A	Abundant expressing cells in the basal magnocellular complex (BMC).
TS24: amygdala	Present	Regionally restricted	3A	Expression throughout the entire diagonal area (Dg)-derived amygdalar nuclei. Expression in most cells in the extended amygdala, sublenticular part (SLEA).
TS24: pallidum	Present	Regionally restricted	3A	Expression only in medial region of bed nucleus of stria terminalis (BSTM).
TS24: globus pallidus	Absent		3A

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3B	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei. Expression in the anterior amygdala, ventral part (Aav). Expression in a few cells in the central amygdala (Ce). Expression in most cells in the extended amygdala (EA).
TS24: basal ganglia	Present	Regionally restricted	3B	Scattered distribution of expression in anterior preoptic area (POA) derivatives such as the nucleus basalis (BAS).
TS24: globus pallidus	Absent		3B

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3C	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei. Expression in scattered cells in the medial amygdala, anterior part (MeA).
TS24: pallidum	Present	Regionally restricted	3C	No expression in pallidal region of subpallium (BSTL).

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3D	Expression only in region derived from the diagonal area (Dg), the anterior and posterodorsal parts (MeA and MePD) of the medial amygdala. Expression in a few cells in the central amygdala (Ce).
TS24: caudate-putamen	Absent		3D

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3E	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei. Expression in most cells in the extended amygdala (EA).

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3F	Expression in most cells in the extended amygdala (EA). Expression only in region derived from the diagonal area (Dg), the anterior and posterodorsal parts (MeA and MePD) of the medial amygdala.

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3G	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei. Expression in a few cells in the central amygdala (Ce). Expression in most cells in the extended amygdala (EA). Expression in scattered cells in the medial amygdala, anterior part (MeA).

Structure	Level	Pattern	Image	Note
TS24: basal forebrain	Present	Regionally restricted	3H	Abundant expressing cells in the basal magnocellular complex (BMC).
TS24: amygdala	Present	Regionally restricted	3H	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei. Expression in scattered cells in the medial amygdala, anterior part (MeA).

Structure	Level	Pattern	Image	Note
TS24: basal forebrain	Present	Regionally restricted	3I	Abundant expressing cells in the basal magnocellular complex (BMC).
TS24: amygdala	Present	Regionally restricted	3I	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei. Expression in a few cells in the central amygdala (Ce). Expression in most cells in the extended amygdala (EA).

Structure	Level	Pattern	Image	Note
TS24: basal forebrain	Present	Regionally restricted	3J	Abundant expressing cells in the basal magnocellular complex (BMC).
TS24: amygdala	Present	Regionally restricted	3J	Expression in a few cells in the central amygdala (Ce). Expression only in region derived from the diagonal area (Dg), including the posterodorsal part (MePD) of the medial amygdala.

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3K	Expression throughout the entire diagonal area (Dg)-derived amygdalar nuclei.
TS24: pallidum	Present	Regionally restricted	3K	Expression only in medial region of bed nucleus of stria terminalis (BSTM).
TS24: caudate-putamen	Absent		3K

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3L	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei.
TS24: pallidum	Present	Regionally restricted	3L	Expression only in medial region of bed nucleus of stria terminalis (BSTM)
TS24: caudate-putamen	Absent		3L

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3M	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei. Expression in a few cells in the central amygdala (Ce). Expression in the anterior amygdala, ventral part (Aav).

Structure	Level	Pattern	Image	Note
TS24: basal forebrain	Present	Regionally restricted	3O	Abundant expressing cells in the basal magnocellular complex (BMC).
TS24: amygdala	Present	Regionally restricted	3O	Expression throughout entire diagonal area (Dg)-derived amygdalar nuclei.

Structure	Level	Pattern	Image	Note
TS25: basal forebrain	Present	Regionally restricted	4A	Scattered distribution of expression in anterior preoptic area (POA) derivatives such as the basal magnocellular complex (BMC).
TS25: pallidum	Present	Regionally restricted	4A	Expression in some cells in the ventral pallidum (VP).
TS25: amygdala	Present	Regionally restricted	4A	Expression in most cells in the extended amygdala (EA).
TS25: caudate-putamen	Absent		4A

Structure	Level	Pattern	Image	Note
TS25: basal forebrain	Present	Regionally restricted	4B	Scattered distribution of expression in anterior preoptic area (POA) derivatives such as the basal magnocellular complex (BMC).
TS25: pallidum	Present	Regionally restricted	4B	Expression in some cells in the ventral pallidum (VP).
TS25: caudate-putamen	Absent		4B

Structure	Level	Pattern	Image	Note
TS25: basal forebrain	Present	Regionally restricted	4C	Scattered distribution of expression in anterior preoptic area (POA) derivatives such as the basal magnocellular complex (BMC).
TS25: amygdala	Present	Regionally restricted	4C	Expression in the extended amygdala, sublenticular part (SLEA).
TS25: pallidum	Present	Regionally restricted	4C	Expression only in medial region of bed nucleus of stria terminalis (BSTM). No expression in lateral region of bed nucleus of stria terminalis (BSTL).

Structure	Level	Pattern	Image	Note
TS25: amygdala	Present	Regionally restricted	4D	Expression in the anterior amygdala, ventral part (AAv). Expression in most cells in the extended amygdala (EA).

Structure	Level	Pattern	Image	Note
TS25: amygdala	Present	Regionally restricted	4E	Expression in the anterior amygdala, ventral part (AAv). Expression in scattered cells in the medial amygdala, anterior part (MeA).

Structure	Level	Pattern	Image	Note
TS25: amygdala	Present	Regionally restricted	4F	Expression only in region derived from the diagonal area (Dg), including the posterodorsal part (MePD) of the medial amygdala.

Structure	Level	Pattern	Image	Note
TS25: amygdala	Present	Regionally restricted	4G	Expression in most cells in the extended amygdala (EA).

Structure	Level	Pattern	Image	Note
TS25: amygdala	Present	Regionally restricted	4H	Expression in most cells in the extended amygdala (EA).

Structure	Level	Pattern	Image	Note
TS25: basal forebrain	Present	Regionally restricted	4I , 4K	Expression in the basal magnocellular complex (BMC).
TS25: amygdala	Present	Regionally restricted	4I , 4K	Expression in the extended amygdala, sublenticular part (SLEA).

Structure	Level	Pattern	Image	Note
TS27: basal magnocellular complex	Present		5A	Many expressing cells in the basal magnocellular complex (BMC).
TS27: amygdala	Present	Regionally restricted	5A	Expression in cells located in the ventromedial extended amygdala (EA).
TS27: pallidum	Present	Regionally restricted	5A	Expression in bed nucleus of stria terminalis, medial part (BSTM).
TS27: globus pallidus	Absent		5A

Structure	Level	Pattern	Image	Note
TS27: basal magnocellular complex	Present		5B	Many expressing cells in the basal magnocellular complex (BMC).
TS27: amygdala	Present	Regionally restricted	5B	Expression in cells located in the ventromedial extended amygdala (EA).
TS27: bed nucleus of stria terminalis	Present	Regionally restricted	5B	Expression in bed nucleus of stria terminalis, medial part (BSTM).

Structure	Level	Pattern	Image	Note
TS27: basal magnocellular complex	Present		5E	Many expressing cells in the basal magnocellular complex (BMC).
TS27: amygdala	Present	Regionally restricted	5E	Few expressing cells in the central amygdala (Ce). Expression in cells located in the ventromedial extended amygdala (EA).
TS27: globus pallidus	Absent		5E

Structure	Level	Pattern	Image	Note
TS27: basal magnocellular complex	Present		5F	Many expressing cells in the basal magnocellular complex (BMC).
TS27: amygdala	Present	Regionally restricted	5F	Few expressing cells in the central amygdala (Ce). Expression in cells located in the ventromedial extended amygdala (EA).
TS27: globus pallidus	Absent		5F

Structure	Level	Pattern	Image	Note
TS27: amygdala	Present	Regionally restricted	5I	Expression in the medial amygdala, anterior part (MeA). Expression in the anterior amygdala, ventral part (AAv). Few expressing cells in the central amygdala (Ce). Expression in cells located in the ventromedial extended amygdala (EA).

Structure	Level	Pattern	Image	Note
TS27: amygdala	Present	Regionally restricted	5J	Expression in the anterior amygdala, ventral part (AAv). Few expressing cells in the central amygdala (Ce).