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Gene Expression Data
Assay Details
Assay
Reference: J:43266 Handler M, et al., Developmental expression of perlecan during murine embryogenesis. Dev Dyn. 1997 Oct;210(2):130-45
Assay type: RT-PCR
MGI Accession ID: MGI:5824223
Gene symbol: Hspg2
Gene name: perlecan (heparan sulfate proteoglycan 2)
Probe: perlecan-pI, perlecan-pJ
Probe preparation: labelled with P32
Visualized with: Autoradiography
Assay notes: Southern blot followed RT-PCR amplication using the probe MGI:5823467.
Results
Image: 7B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 351 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex
E13 E13.0 TS21: embryo Present 5-8 µg; total RNA Not Specified Not Specified
E14 E14.0 TS22: embryo Present 5-8 µg; total RNA Not Specified Not Specified
E15 E15.0 TS23: embryo Present 5-8 µg; total RNA Not Specified Not Specified
E18 E18.0 TS26: embryo Present 5-8 µg; total RNA Not Specified Not Specified
Heart P adult TS28: heart Strong 5-8 µg; total RNA Not Specified Not Specified
Colon P adult TS28: colon Strong 5-8 µg; total RNA Not Specified Not Specified
Testis P adult TS28: testis Strong 5-8 µg; total RNA Not Specified Male
Liver P adult TS28: liver Present 5-8 µg; total RNA Not Specified Not Specified
Cartilage P adult TS28: cartilage Present 5-8 µg; total RNA Not Specified Not Specified
Thymus P adult TS28: thymus Present 5-8 µg; total RNA Not Specified Not Specified
Kidney P adult TS28: metanephros Present 5-8 µg; total RNA Not Specified Not Specified
Salivary Gland P adult TS28: salivary gland Present 5-8 µg; total RNA Not Specified Not Specified
Ovary P adult TS28: ovary Weak 5-8 µg; total RNA Not Specified Female
Muscle P adult TS28: skeletal muscle Weak 5-8 µg; total RNA Not Specified Not Specified
Brain P adult TS28: brain Trace 5-8 µg; total RNA Not Specified Not Specified
Notes:
(a) Expression level increased as embryonic development progressed.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory