Probe:	NAIP cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	1a	Expression is surrounding the 4th ventricle.
TS15: future brain	Present	Regionally restricted	1a	Expression is rostral.
TS15: future midbrain lateral wall	Present		1a
TS15: neural tube	Present		1a

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	1b	Expression is surrounding the mesencephalic vesicle.
TS19: 1st branchial arch	Present	Regionally restricted	1b	Expression is at the periphery.
TS19: 2nd branchial arch	Present	Regionally restricted	1b	Expression is at the periphery.
TS19: telencephalon	Present	Regionally restricted	1b	Expression is at the periphery.
TS19: future spinal cord	Present		1b
TS19: hindbrain	Present		1b

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		1c
TS20: diencephalon	Present		1c
TS20: forelimb digit	Present		1c
TS20: hindlimb digit	Present		1c
TS20: medulla oblongata	Present		1c
TS20: midbrain	Present		1c
TS20: nasal epithelium	Present		1c
TS20: spinal cord mantle layer	Present		1c

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present		1d
TS21: medulla oblongata	Present		1d
TS21: midbrain	Present		1d
TS21: nasal epithelium	Present		1d
TS21: pons	Present		1d
TS21: spinal cord mantle layer	Present		1d
TS21: tongue	Present		1d

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit	Present		1e
TS20: forelimb epithelium	Present	Regionally restricted	1e	Expression is at the dorsal surface.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		1f
TS21: spinal cord mantle layer	Present	Regionally restricted	1f	Expression is in the lumbo-sacral layer.

Structure	Level	Pattern	Image	Note
TS17: brain	Present
TS17: hindbrain	Present
TS17: midbrain	Present
TS17: neural tube	Present