Summary | Mutation origin | Mutation description | Expression | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1(CAG-HIST1H2BJ/mCherry,-EGFP/Rpl10a)Evdr
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Evan Rosen
MGI ID:	MGI:5817798
Synonyms:	H2B-TRAP
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1(CAG-HIST1H2BJ/mCherry,-EGFP/Rpl10a)Evdr page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The targeting vector was designed with (from 5' to 3') with a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a FRT site, a loxP-flanked STOP, the H2B-mCherry fusion protein (coding sequence for the human histone 1 H2bj gene [HIST1H2BJ] fused in-frame to the N-terminus of a monomeric red fluorescent protein sequence [mCherry]), a 2A peptide, the EGFP-L10a fusion protein (an enhanced green fluorescent protein gene [EGFP] fused in-frame to the N-terminus of a cDNA sequence encoding the mouse 60S ribosomal subunit L10a [Rpl10a]), WPRE,a polyA signal, an AttB site, PGK-FRT-Neo-PGK polyA), and an AttP site. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:101977)

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	2 reference(s)