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Gene Expression Data
Assay Details
Assay
Reference: J:77015 Deng W, et al., miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell. 2002 Jun;2(6):819-30
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5816726
Gene symbol: Piwil1
Gene name: piwi-like RNA-mediated gene silencing 1
Antibody: anti-R133
Detection system: Secondary antibody
Results
Specimen 3E: postnatal day 14 (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3E Expression is in pachytene spermatocytes.
TS28: interstitium of the testis Absent 3E
TS28: Sertoli cell Sertoli cell Absent 3E

Specimen 3F: postnatal adult (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present 3F Expression is in pachytene stage and diplotene stage spermatocytes.
TS28: spermatid Present 3F Expression is in round and elongated spermatids.
TS28: interstitium of the testis Absent 3F
TS28: Sertoli cell Sertoli cell Absent 3F

Specimen 3G: postnatal adult (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present 3G Expression is in pachytene stage and diplotene stage spermatocytes.
TS28: spermatid Present 3G Expression is in round and elongated spermatids.
TS28: interstitium of the testis Absent 3G
TS28: Sertoli cell Sertoli cell Absent 3G

Specimen 3H: postnatal adult (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present 3H Expression is in pachytene stage and diplotene stage spermatocytes.
TS28: spermatid Present 3H Expression is in round and elongated spermatids.
TS28: interstitium of the testis Absent 3H
TS28: Sertoli cell Sertoli cell Absent 3H

Specimen 3I: postnatal adult (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present 3I Expression is in pachytene stage and diplotene stage spermatocytes.
TS28: spermatid Present 3I Expression is in round and elongated spermatids.
TS28: interstitium of the testis Absent 3I
TS28: Sertoli cell Sertoli cell Absent 3I

Specimen 3J: postnatal adult; Piwil1tm1Hfl/Piwil1tm1Hfl (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Level Pattern Image Note
TS28: spermatid Absent 3J
TS28: spermatocyte Absent 3J

Specimen 6F: postnatal (more )
Structure Level Pattern Image Note
TS28: testis Present 6F

Specimen 6G: postnatal; Cremtm1Saco/Cremtm1Saco (more )
Structure Level Pattern Image Note
TS28: testis Present 6G Expression in this mutant is the same as in wild-type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory