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Gene Expression Data
Assay Details
Assay
Reference: J:77015 Deng W, et al., miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell. 2002 Jun;2(6):819-30
Assay type: RNA in situ
MGI Accession ID: MGI:5816725
Gene symbol: Piwil1
Gene name: piwi-like RNA-mediated gene silencing 1
Probe: Miwi cDNA5
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3A: postnatal day 12 (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Level Pattern Image Note
TS28: germ cell of testis Present 3A Expression is in germ cells in the central region of the tubules.
TS28: seminiferous tubule Present Regionally restricted 3A Expression is in a subset of tubules.
Specimen 3B: postnatal day 14 (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Level Pattern Image Note
TS28: germ cell of testis Present 3B Expression is in germ cells in the luminal region of the tubules.
TS28: seminiferous tubule Present Regionally restricted 3B Expression is in a subset of tubules.
Specimen 3C: postnatal adult (more )
Note: Double labeled: red - Piwil1; green - laminin.
Structure Cell Type Level Pattern Image Note
TS28: primary spermatocyte Present Regionally restricted 3C Most expressing cells are one cell away from the basement membrane, with a small number at the basal layer.
TS28: spermatocyte Present Single cells 3C Expression is in zygotene stage spermatocytes.
TS28: seminiferous tubule Present Regionally restricted 3C Expression is in a subset of tubules.
TS28: Sertoli cell Sertoli cell Absent 3C
Specimen 3D: postnatal adult (more )
Note: Double labeled: red - Piwil1; green - Tsx (assay MGI:5816886).
Structure Cell Type Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 3D Expression is in a subset of tubules.
TS28: Sertoli cell Sertoli cell Absent 3D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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