Gene Expression Data
Assay Details
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Assay
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Reference:
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J:145505
Stiber J, et al., STIM1 signalling controls store-operated calcium entry required for development and contractile function in skeletal muscle. Nat Cell Biol. 2008 Jun;10(6):688-97
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5811513
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Gene symbol:
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Stim1
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Gene name:
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stromal interaction molecule 1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Electron microscopy
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Results
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Specimen
4d:
postnatal adult; Stim1Gt(RRS558)Byg/Stim1 +
(more  )
Note: Aggregates of beta-glactosidase products were examined by electron microscopy. Specimens were post-fixed in 2% paraformaldehyde and 2% glutaraldehyde, fixed in 1% OSO4 and embedded in an epoxy resin mixture.
Specimen
4d:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Stim1Gt(RRS558)Byg/Stim1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Aggregates of beta-glactosidase products were examined by electron microscopy. Specimens were post-fixed in 2% paraformaldehyde and 2% glutaraldehyde, fixed in 1% OSO4 and embedded in an epoxy resin mixture.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hindlimb musculature |
Present |
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4d
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Expression is in the longitudinal sarcoplasmic reticulum as well as the junction of the t-tubule and terminal sarcoplasmic reticulum.
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Specimen
4e:
postnatal adult; Stim1Gt(RRS558)Byg/Stim1 +
(more )
Note: Aggregates of beta-glactosidase products were examined by electron microscopy. Specimens were post-fixed in 2% paraformaldehyde and 2% glutaraldehyde, fixed in 1% OSO4 and embedded in an epoxy resin mixture.
Specimen
4e:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Stim1Gt(RRS558)Byg/Stim1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Aggregates of beta-glactosidase products were examined by electron microscopy. Specimens were post-fixed in 2% paraformaldehyde and 2% glutaraldehyde, fixed in 1% OSO4 and embedded in an epoxy resin mixture.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hindlimb musculature |
Present |
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4e
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Expression is in the longitudinal sarcoplasmic reticulum as well as the junction of the t-tubule and terminal sarcoplasmic reticulum.
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Specimen
S3A:
postnatal adult; Stim1Gt(RRS558)Byg/Stim1 +
(more )
Note: (B) is a higher magnification of the boxed region in (A).
Specimen
S3A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Stim1Gt(RRS558)Byg/Stim1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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(B) is a higher magnification of the boxed region in (A).
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Specimen
S3C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Stim1Gt(RRS558)Byg/Stim1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S3D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Stim1Gt(RRS558)Byg/Stim1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S3E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Stim1Gt(RRS558)Byg/Stim1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spleen |
Present |
Regionally restricted |
S3E
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Expression is in a subset of cells.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Stim1Gt(RRS558)Byg/Stim1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: thymus |
Present |
Regionally restricted |
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Expression is in a subset of cells.
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Analysis Tools