Gene Expression Data
Assay Details
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Assay
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Reference:
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J:217681
Halstead AM, et al., Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning. Mech Dev. 2015 May;136:155-65
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5811489
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Gene symbol:
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Lefty2
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Gene name:
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left-right determination factor 2
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Probe:
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Lefty2 probe10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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Either wild type or Foxh1F/F (MGI:5780728) embryos were used as controls. Authors assert that Foxh1F/F embryos were molecularly and morphologically identical to wild type.
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Results
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Specimen
2C ctrl:
embryonic day 6.75
(more  )
Specimen
2C ctrl:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Age:
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embryonic day 6.75
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
2D mut:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Regionally restricted |
2D
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Authors state expression was similar between control and mutant embryos. No spatial or temporal defects in the expression.
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Specimen
3C ctrl:
embryonic day 8.25
(more )
Specimen
3C ctrl:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
3D mut:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3D
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Authors report no aberrant expression of L-R patterning gene detected in mutant embryos.
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Specimen
S4E ctrl:
embryonic day 8.25
(more )
Specimen
S4E ctrl:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
S4F mut:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
S4F
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Authors report lack of ectopic expression in the right lateral plate mesoderm.
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Specimen
S4G ctrl:
embryonic day 8.25
(more )
Specimen
S4G ctrl:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * SJL
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
S4H mut:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
S4H
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Authors report lack of ectopic expression in the right lateral plate mesoderm.
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Analysis Tools