Probe:	probe C
Probe preparation:	Double stranded labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present	Regionally restricted	5A	Expression in endothelium lining the lumen.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	5B , 5C	Expression in both capillaries and connective tissue of meninges.

Structure	Level	Pattern	Image	Note
TS27: blood vessel endothelium	Strong		5D

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	5E

Structure	Level	Pattern	Image	Note
TS26: brain meninges	Present		5F
TS26: head mesenchyme	Present	Regionally restricted	5F	Expression in connective tissue of head.

Structure	Level	Pattern	Image	Note
TS23: dermis connective tissue	Present	Regionally restricted	5G	Expression predominantly in dense connective tissue.
TS23: rib	Present	Regionally restricted	5G	Expression in perichondrium of ribs.
TS23: pericardium	Present		5G

Structure	Level	Pattern	Image	Note
TS24: parietal pericardium	Present		5H
TS24: visceral pericardium	Present		5H

Structure	Level	Pattern	Image	Note
TS24: tooth mesenchyme	Strong	Regionally restricted	5I	Strong expression in dental mesenchyme of both cuspid and pulp.
TS24: alveolar sulcus	Present		5I	Expression in the alveolar bone.

Structure	Level	Pattern	Image	Note
TS24: vibrissa	Present	Regionally restricted	5J	Expression in connective tissue that surrounds each vibrissa.

Structure	Level	Pattern	Image	Note
TS24: trunk mesenchyme	Present	Regionally restricted	5K	Expression in differentiating fibroblasts adjacent to the vertebral column. Authors report expression in ligaments.

Structure	Level	Pattern	Image	Note
TS24: intestine muscularis	Present	Regionally restricted	5L	Expression in smooth muscle layer of some, but not all, segments of the intestine.

Structure	Level	Pattern	Image	Note
TS24: renal medulla	Present		5M	Strong expression in central mesenchyme of kidney. Expression restricted to the medullary zone.

Structure	Level	Pattern	Image	Note
TS24: dermis reticular layer	Present	Regionally restricted	5N	Significant expression in reticular dermis.

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle	Present		5O	Expression in differentiating skeletal muscle.

Structure	Level	Pattern	Image	Note
TS26: perichondrium	Present		6B
TS26: tendon	Present		6B
TS26: long bone	Trace	Regionally restricted	6B	Scant expression in chondrocytes. Low levels of expression in the late proliferative zone. Low levels of expression in osteoblasts associated with newly-deposited osteoid.

Structure	Level	Pattern	Image	Note
TS26: bone	Present	Regionally restricted	6E	Low levels of expression in the late proliferative zone.

Structure	Level	Pattern	Image	Note
TS25: vertebra	Present	Regionally restricted	6H	Expression in periosteum.

Structure	Level	Pattern	Image	Note
TS24: rib	Present	Regionally restricted	6K	Expression in connective tissue of perichondrium and periosteum.

Structure	Level	Pattern	Image	Note
TS25: tail	Present	Regionally restricted	6N	Expression in connective tissue of perichondrium and periosteum.

Structure	Level	Pattern	Image	Note
TS24: lung	Present		7B	Expression at the beginning of canalicular phase as lung is invaded by capillaries (day 16).
TS24: bronchus	Present	Regionally restricted	7B	Expression in mucosa of developing bronchi.
TS24: parietal pleura	Present		7B
TS24: visceral pleura	Present		7B
TS24: rib	Present	Regionally restricted	7B	Expression in perichondrium of ribs.

Structure	Level	Pattern	Image	Note
TS24: lung	Present		7E	Expression at the beginning of canalicular phase as lung is invaded by capillaries (day 16).
TS24: bronchus mesenchyme	Present		7E
TS24: bronchus epithelium	Absent		7E

Structure	Level	Pattern	Image	Note
TS26: lung mesenchyme	Present	Regionally restricted		Expression in cells beneath epithelium of bronchi and bronchioles.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Absent		8B

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Absent		8E

Structure	Level	Pattern	Image	Note
TS27: accessory olfactory bulb	Absent		8H

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Absent		8K

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present		8N	Expression primarily in granular cells.

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Absent		8Q