Smad2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218065 Hayata T, et al., Dullard/Ctdnep1 Regulates Endochondral Ossification via Suppression of TGF-beta Signaling. J Bone Miner Res. 2015 Feb;30(2):318-29
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5796647
Gene symbol:	Smad2
Gene name:	SMAD family member 2

Antibody:	anti-phospho-Smad2/3 (9510)
Assay notes:	The antibody recognizes the phosphorylated form of the protein. Both Smad2 and Smad3 are annotated. A TSA-biotin system was used for detection.

Results

Specimen 5 Digit WT: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: digit mesenchyme	Present	Regionally restricted	5_Digit WT	Expression is in the perichondrium.

Specimen 5 Digit KO: embryonic day 13.5; Ctdnep1^tm3Ryn/Ctdnep1^tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS21: digit mesenchyme	Very strong	Regionally restricted	5_Digit KO	Expression is in the perichondrium.

Specimen 5 Humerus WT: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: humerus	Present	Regionally restricted	5_Humerus WT	Expression is in the prehypertrophic and hypertrophic chondrocytes in the growth plate.

Specimen 5 Humerus KO: embryonic day 15.5; Ctdnep1^tm3Ryn/Ctdnep1^tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: humerus	Very strong	Regionally restricted	5_Humerus KO	Expression is in the prehypertrophic and hypertrophic chondrocytes in the growth plate.

Specimen S10 Sternum WT: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: sternum	Present		S10_Sternum WT

Specimen S10 Sternum KO: embryonic day 15.5; Ctdnep1^tm3Ryn/Ctdnep1^tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: sternum	Present		S10_Sternum KO	Expression in this mutant is the same as in wild-type embryo.

Specimen S10 Metacarpal WT: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: metacarpal bone	Present		S10_Metacarpal WT

Specimen S10 Metacarpal KO: embryonic day 15.5; Ctdnep1^tm3Ryn/Ctdnep1^tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: metacarpal bone	Present		S10_Metacarpal KO	Expression in this mutant is the same as in wild-type embryo.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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