About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:137253 Plachez C, et al., Nuclear factor I gene expression in the developing forebrain. J Comp Neurol. 2008 May 20;508(3):385-401
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5792128
Gene symbol: Nfib
Gene name: nuclear factor I/B
Antibody: Anti-NFIB
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1J: postnatal; Nfibtm1Rmg/Nfibtm1Rmg (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS28: brain Absent 1J
Specimen 1K: postnatal (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS28: brain Present 1K
Specimen 2J/J': embryonic day 18.5 (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS26: neocortex Present 2J' , 2J
TS26: cerebral cortex subventricular zone Present 2J' , 2J Expressed at lower levels compared to in the neocortex.
TS26: cerebral cortex ventricular zone Present 2J' , 2J Expressed at lower levels compared to in the neocortex.
Specimen 5F/F'/F": postnatal day 5 (more )
Note: Vibratome section. Double labeled: red - Nfib; green - Rbfox3 (assay MGI:5792535).
Structure Level Pattern Image Note
TS28: cortical layer V Present 5F merge , 5F left , 5F' merge , 5F' left Most positive cells colocalized with Rbfox3.
TS28: cortical layer VI Present 5F" left , 5F left , 5F" merge , 5F merge Most positive cells colocalized with Rbfox3.
Specimen 5H/H'/H": embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Double labeled: red - Nfib; green - Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: cerebral cortex marginal zone Present 5H , 5H' The majority of the expression did not colocalize with Gad1 (GABAergic neurons). A subpopulation of cells coexpressed both.
TS26: cortical plate Present 5H , 5H" The majority of the expression did not colocalize with Gad1 (GABAergic neurons). A small number of cells coexpressed both.
Specimen 9F/F': embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Double labeled: red - Nfib; green - Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: prethalamus Present Scattered 9F' , 9F A small number of the cells were double-labeled with GAD1.
TS26: diencephalon Present Regionally restricted 9F' Expressed in cells lining the third ventricle.
TS26: hypothalamus Present 9F
Specimen not shown: embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Also stained for Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: dentate gyrus Present Expression did not colocalize with Gad1 (GABAergic neurons).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory