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Gene Expression Data
Assay Details
Assay
Reference: J:169043 Chapman G, et al., Notch inhibition by the ligand Delta-Like 3 defines the mechanism of abnormal vertebral segmentation in spondylocostal dysostosis. Hum Mol Genet. 2011 Mar 1;20(5):905-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5790068
Gene symbol: Dll3
Gene name: delta like canonical Notch ligand 3
Antibody: DLL3 antibody
Detection system: Secondary antibody
Results
Specimen 1H: embryonic day 9.5 (more )
Note: Freshly dissected unfixed non-permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Absent 1H Dll3 Absent in the non-permeabilized specimen (antibody is unable to penetrate the cell).
Specimen 1I: embryonic day 9.5 (more )
Note: Freshly dissected unfixed permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 1I Dll3 Expression is in the presomitic mesoderm in this permabilized specimen.
Specimen 2D: embryonic day 9.5 (more )
Note: Double labeled: green - Dll3; red - Lamp1 (assay MGI:5790070).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 2D Dll3 , 2D merge Expression is in the caudal presomitic mesoderm.
Specimen 2I: embryonic day 9.5 (more )
Note: Triple labeled: blue- Dll3; green - Notch1 (assay MGI:5790067); red - Lamp1 (assay MGI:5790070).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 2I merge , 2I Dll3 Expression is in the rostral presomitic mesoderm.
Specimen 3A: embryonic day 9.5 (more )
Note: (B) is a higher magnification of the boxed area in (A).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Punctate 3A , 3B Expression is in the rostral presomitic mesoderm.
Specimen 3C: embryonic day 9.5 (more )
Note: Double labeled: red - Dll3; green - Golga2 (assay MGI:5790065).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Punctate 3C Dll3 , 3C merge Expression is in the rostral presomitic mesoderm.
Specimen 3D: embryonic day 9.5 (more )
Note: Double labeled: green - Dll3; red - Epha4 (assay MGI:5790072).
Structure Level Pattern Image Note
TS15: somite Present Regionally restricted 3D Dll3 , 3D merge Expression is in the rostral presomitic mesoderm throughout the somite-sized region caudal to the forming somite up to the position of the presumptive somite boundary.
TS15: tail unsegmented mesenchyme Present Regionally restricted 3D Dll3 , 3D merge Expression is in the rostral presomitic mesoderm throughout the somite-sized region caudal to the forming somite up to the position of the presumptive somite boundary.
Specimen 3G: embryonic day 9.5 (more )
Note: Double labeled: red - Dll3; green - Notch1 (assay MGI:5790067).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3G Dll3 , 3G merge Expression is in the rostral presomitic mesoderm. Expression is throughout the somite-sized region caudal to the forming somite up to the position of the presumptive somite boundary.
Specimen 3J: embryonic day 9.5 (more )
Note: Triple labeled: blue- Dll3; red - Notch1 (assay MGI:5790067); green - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3J Expression is in the rostral presomitic mesoderm.
Specimen 3K: embryonic day 9.5 (more )
Note: Inset is a higher magnification of the boxed region. Triple labeled: red - Dll3; green - Notch1 (assay MGI:5790067); blue - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3K Dll3 , 3K merge Expression is in the rostral presomitic mesoderm.
Specimen 3L: embryonic day 9.5 (more )
Note: Inset is a higher magnification of the boxed region. Triple labeled: blue - Dll3; red - Notch1 (assay MGI:5790067); green - Golga2 (assay MGI:5790065).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3L Dll3 , 3L merge Expression is in the rostral presomitic mesoderm.
Specimen 4E: embryonic day 9.5 (more )
Note: (G and H) are higher magnifications of (E). Triple labeled: green - Dll3; blue - Dll1 (assay MGI:5790062); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4G Dll3 , 4E Dll3 , 4G merge , 4E merge , 4H Expression is in the rostral presomitic mesoderm.
Specimen 4I: embryonic day 9.5; Dll3tm1Rbe/Dll3tm1Rbe (more )
Note: (J) is a higher magnification of (I). Triple labeled: green - Dll3; red - cleaved Notch1 (assay MGI:5790075); blue - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS15: trunk paraxial mesenchyme Absent 4J merge , 4I Dll3 , 4J Dll3 , 4I merge
Specimen S2: embryonic day 9.5 (more )
Note: Image analysis of Figure 2I. Triple labeled: blue- Dll3; green - Notch1 (assay MGI:5790067); red - Lamp1 (assay MGI:5790070).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S2i , S2g , S2h , S2f Expression is in the rostral presomitic mesoderm.
Specimen S3a: embryonic day 9.5 (more )
Note: Triple labeled: green - Dll3; blue - Dll1 (assay MGI:5790062); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S3a Dll3 , S3a merge Expression is in the rostral presomitic mesoderm.
Specimen S3b: embryonic day 9.5 (more )
Note: Triple labeled: green - Dll3; blue - Dll1 (assay MGI:5790062); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S3b merge , S3b Dll3 Expression is in the rostral presomitic mesoderm.
Specimen S3c: embryonic day 11.5 (more )
Note: Triple labeled: green - Dll3; blue - Dll1 (assay MGI:5790062); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present Regionally restricted S3c merge , S3c Dll3 Expression is in the rostral presomitic mesoderm.
Specimen S3d: embryonic day 11.5 (more )
Note: Triple labeled: green - Dll3; blue - Dll1 (assay MGI:5790062); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present Regionally restricted S3d merge , S3d Dll3 Expression is in the rostral presomitic mesoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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