Antibody:	anti-Fibronectin (ab2033)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted	1F	Expression localized between neural epithelium containing neural crest progenitors and non-neural surface ectoderm.

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present		S1A

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted	S1A1	Expression between neural surface ectoderm and cardiac neural crest progenitors.

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present	Regionally restricted	S1A2	Expression surrounding mesenchymal cardiac neural crest cells.

Structure	Level	Pattern	Image	Note
TS13: branchial arch mesenchyme	Absent		S1B	Authors report minimal staining.
TS13: surface ectoderm	Absent		S1B	Authors report minimal staining.