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Gene Expression Data
Assay Details
Assay
Reference: J:153908 de Castro BM, et al., The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009 Oct;29(19):5238-50
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5788427
Gene symbol: Slc5a7
Gene name: solute carrier family 5 (choline transporter), member 7
Antibody: Anti-Cht1
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 6B wt/wt: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: diaphragm Present 6B CHT1 wt/wt
Specimen 6B wt/del: embryonic day 18.5; Chat/Slc18a3tm1.1Vpra/Chat+, Chat/Slc18a3tm1.1Vpra/Slc18a3+ (more )
Note: This embryo was a single heterozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double heterozygote).
Structure Level Pattern Image Note
TS26: diaphragm Present 6B CHT1 wt/del
Specimen 6B del/del: embryonic day 18.5; Chat/Slc18a3tm1.1Vpra/Chat/Slc18a3tm1.1Vpra, Chat/Slc18a3tm1.1Vpra/Chat/Slc18a3tm1.1Vpra (more )
Note: This embryo was a single homozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Structure Level Pattern Image Note
TS26: diaphragm Present 6B CHT1 del/del Immunolabeling was detected in the mutant.
Specimen 7A wt/wt: embryonic day 18.5 (more )
Note: Quantification of immunofluorescence.
Structure Level Pattern Image Note
TS26: diaphragm Present 7A wt/wt
Specimen 7A wt/del: embryonic day 18.5; Chat/Slc18a3tm1.1Vpra/Chat+, Chat/Slc18a3tm1.1Vpra/Slc18a3+ (more )
Note: Quantification of immunofluorescence. The embryos contributing to these data were single heterozygotes. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animals looking like a double heterozygotes).
Structure Level Pattern Image Note
TS26: diaphragm Present 7A wt/del
Specimen 7A del/del: embryonic day 18.5; Chat/Slc18a3tm1.1Vpra/Chat/Slc18a3tm1.1Vpra, Chat/Slc18a3tm1.1Vpra/Chat/Slc18a3tm1.1Vpra (more )
Note: Quantification of immunofluorescence. The embryos contributing to these data were single homozygotes. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animals looking like a double homozygotes).
Structure Level Pattern Image Note
TS26: diaphragm Present 7A del/del Immunoreactivity increased at the neuromuscular junction.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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