Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO/Prnp-GLRA1*,-EYFP)9531Jjan
Name:	transgene insertion 9531, Joanna L Jankowsky
MGI ID:	MGI:5779815
Synonyms:	TRE-GlyCl 9531
Transgene:	Tg(tetO/Prnp-GLRA1*,-EYFP)9531Jjan  Location: unknown
Alliance:	Tg(tetO/Prnp-GLRA1*,-EYFP)9531Jjan page

Strain of Origin:

(C57BL/6J x C3H/HeJ)F2

Transgene Type:    Transgenic (Humanized sequence, Inducible, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: The transgene has a Tet-responsive element 3G promoter and the mouse prion protein non-coding exons 1-2 upstream of a cassette containing an enhanced yellow fluorescent reporter (EYFP) flanked on either side by a human glycine receptor alpha1 (GLRA1) cDNA sequence harboring the point mutations F207A (to eliminate endogenous ligand [glycine] sensitivity) and A288G (to increase ivermectin sensitivity almost 100-fold), followed by the mouse prion protein non-coding exon 3 and the woodchuck hepatitis post-transcriptional regulatory element (WPRE). To achieve tricistronic expression of the point mutations and EYFP, they are separated by T2A peptide sequences (from Thosea asigna virus). Lines 9531 and 9542 displayed the strongest signal. Line 9531 is recommended by the donating investigator for most experimental purposes as it displays less tTA-independent expression in the cerebellum than line 9542. (J:232624)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:232624 Zhao R, et al., Impaired Recall of Positional Memory following Chemogenetic Disruption of Place Field Stability. Cell Rep. 2016 Jul 19;16(3):793-804
All:	2 reference(s)