Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO/Prnp-GLRA1*,-EYFP)9492Jjan
Name:	transgene insertion 9492, Joanna L Jankowsky
MGI ID:	MGI:5779807
Synonyms:	TRE-GlyCl 9492
Transgene:	Tg(tetO/Prnp-GLRA1*,-EYFP)9492Jjan  Location: unknown
Alliance:	Tg(tetO/Prnp-GLRA1*,-EYFP)9492Jjan page

Strain of Origin:

(C57BL/6J x C3H/HeJ)F2

Transgene Type:    Transgenic (Humanized sequence, Inducible, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: The transgene has a Tet-responsive element 3G promoter and the mouse prion protein non-coding exons 1-2 upstream of a cassette containing an enhanced yellow fluorescent reporter (EYFP) flanked on either side by a human glycine receptor alpha1 (GLRA1) cDNA sequence harboring the point mutations F207A (to eliminate endogenous ligand [glycine] sensitivity) and A288G (to increase ivermectin sensitivity almost 100-fold), followed by the mouse prion protein non-coding exon 3 and the woodchuck hepatitis post-transcriptional regulatory element (WPRE). To achieve tricistronic expression of the point mutations and EYFP, they are separated by T2A peptide sequences (from Thosea asigna virus). (J:232624)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:232624 Zhao R, et al., Impaired Recall of Positional Memory following Chemogenetic Disruption of Place Field Stability. Cell Rep. 2016 Jul 19;16(3):793-804
All:	1 reference(s)