Gene Expression Data
Assay Details
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Assay
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Reference:
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J:192634
Kogo H, et al., HORMAD2 is essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity. Genes Cells. 2012 Nov;17(11):897-912
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5776045
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Gene symbol:
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Hormad2
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Gene name:
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HORMA domain containing 2
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Antibody:
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anti-HORMAD2_C
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1A:
postnatal adult
(more  )
Note: Double labeled: red - Hormad2; blue - DAPI.
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Hormad2; blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: testis |
Present |
Regionally restricted |
1A
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Expression observed as a single fiber-like structures, presumably indicative of pair of sex chromosomes, in late zygotene, pachytene and early diplotene spermatocytes. Intensity peaks at pachytene stage and becomes faint at late diplotene stage. Expression barely detectable in leptotene and early zygotene nuclei.
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Specimen
1A inset:
postnatal adult
(more )
Note: Double labeled: red - Hormad2; blue - DAPI.
Specimen
1A inset:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Hormad2; blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
1A inset
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Expression in pachytene spermatocytes.
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Specimen
2G Zygotene Hormad2-/-:
postnatal adult; Hormad2tm1Kura/ Hormad2tm1Kura
(more )
Note: Zygotene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
Specimen
2G Zygotene Hormad2-/-:
(close )
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Genetic Background:
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either: B6.Cg-Hormad2tm1Kura or (involves: C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj)
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Mutant Allele(s):
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Hormad2tm1Kura/Hormad2tm1Kura
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Zygotene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
2G Zygotene Hormad2-/-
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Expression in many chromosomal fibers.
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Specimen
2G Pachytene-1 Hormad2-/-:
postnatal adult; Hormad2tm1Kura/ Hormad2tm1Kura
(more )
Note: Very early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
Specimen
2G Pachytene-1 Hormad2-/-:
(close )
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Genetic Background:
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either: B6.Cg-Hormad2tm1Kura or (involves: C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj)
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Mutant Allele(s):
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Hormad2tm1Kura/Hormad2tm1Kura
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Very early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
2G Pachytene-1 Hormad2-/-
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Expression in only one chromosomal fiber. Little nucleoplasmic expression.
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Specimen
2G Pachytene-2 Hormad2-/-:
postnatal adult; Hormad2tm1Kura/ Hormad2tm1Kura
(more )
Note: Early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
Specimen
2G Pachytene-2 Hormad2-/-:
(close )
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Genetic Background:
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either: B6.Cg-Hormad2tm1Kura or (involves: C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj)
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Mutant Allele(s):
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Hormad2tm1Kura/Hormad2tm1Kura
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
2G Pachytene-2 Hormad2-/-
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Expression in only one chromosomal fiber. Intense nucleoplasmic expression.
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Specimen
2G Zygotene Wild-type:
postnatal adult
(more )
Note: Zygotene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
Specimen
2G Zygotene Wild-type:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Zygotene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
2G Zygotene Wild-type
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Expression in many chromosomal fibers.
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Specimen
2G Pachytene-1 Wild-type:
postnatal adult
(more )
Note: Very early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
Specimen
2G Pachytene-1 Wild-type:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Very early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
2G Pachytene-1 Wild-type
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Expression in only one chromosomal fiber. Little nucleoplasmic expression.
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Specimen
2G Pachytene-2 Wild-type:
postnatal adult
(more )
Note: Early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
Specimen
2G Pachytene-2 Wild-type:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
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Note:
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Early pachytene stage spermatocytes. Triple labeled: red - Hormad2; green - Atr (assay MGI:5776046); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
2G Pachytene-2 Wild-type
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Expression in only one chromosomal fiber. Intense nucleoplasmic expression.
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Analysis Tools