Gene Expression Data
Assay Details
Assay
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Reference:
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J:65596
Voss AK, et al., Taube nuss is a novel gene essential for the survival of pluripotent cells of early mouse embryos. Development. 2000 Dec;127(24):5449-61
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5775401
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Gene symbol:
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Taf8
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Gene name:
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TATA-box binding protein associated factor 8
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
3A het:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
3A hom:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Weak |
Widespread |
3A
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Staining is seen in all blastomeres. Staining in 21% of the embryos from the cross have double intensity; these are presumed to be the homozygotes.
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Specimen
5A het:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS4: inner cell mass |
Present |
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5A
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Staining in the inner cell mass is more intense than in the trophectoderm.
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TS4: trophectoderm |
Present |
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5A
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Staining in the inner cell mass is more intense than in the trophectoderm.
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Specimen
5a hom:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS4: inner cell mass |
Present |
|
5A
|
Staining in the inner cell mass is more intense than in the trophectoderm. Staining in 21% of the embryos from the cross have double intensity; these are presumed to be the homozygotes.
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TS4: trophectoderm |
Present |
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5A
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Staining in the inner cell mass is more intense than in the trophectoderm. Staining in 21% of the embryos from the cross have double intensity; these are presumed to be the homozygotes.
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Specimen
not shown:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Weak |
Ubiquitous |
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Staining is observed in all tissues.
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Specimen
not shown:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Weak |
Ubiquitous |
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Staining is observed in all tissues.
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Specimen
3C:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Weak |
Ubiquitous |
3C
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Staining is observed in all tissues.
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Specimen
3G:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS19: embryo |
Weak |
Widespread |
3G
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TS19: heart |
Present |
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3G
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Staining in the heart is more intense than in other tissues.
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Specimen
not shown:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
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Staining in the brain is seen primarily in the hippocampus. It is not seen in tissues outside the brain.
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