Gene Expression Data
Assay Details
Assay
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Reference:
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J:65596
Voss AK, et al., Taube nuss is a novel gene essential for the survival of pluripotent cells of early mouse embryos. Development. 2000 Dec;127(24):5449-61
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5775389
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Gene symbol:
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Taf8
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Gene name:
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TATA-box binding protein associated factor 8
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Probe:
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probe 2
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Probe preparation:
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Antisense labelled with radioactivity RNA
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Visualized with:
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Autoradiography
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Assay notes:
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The embryos used for these studies were derived from crosses using one or two heterozygotic parents. Since the heterozygotes exhibit a wild type phenotype, it is possible some of these embryos are heterozygotic (rather than wild type).
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Results
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Specimen
3B:
embryonic day 6.5
(more )
Specimen
3B:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Weak |
Ubiquitous |
3B
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Expression is observed in all tissues.
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Specimen
not shown:
embryonic day 7.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Weak |
Ubiquitous |
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Expression is observed in all tissues.
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Specimen
3D/E:
embryonic day 8.5
(more )
Specimen
3D/E:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Weak |
Ubiquitous |
3D/E
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Expression is observed in all tissues.
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Specimen
3H/I:
embryonic day 11.5
(more )
Specimen
3H/I:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3J/K:
postnatal adult
(more )
Specimen
3J/K:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Weak |
Widespread |
3J/K
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TS28: hippocampus |
Present |
Widespread |
3J/K
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Slightly higher expression levels are observed in the hippocampus relative to other areas of the brain.
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