Chd7 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:231044 Gage PJ, et al., Mouse Models for the Dissection of CHD7 Functions in Eye Development and the Molecular Basis for Ocular Defects in CHARGE Syndrome. Invest Ophthalmol Vis Sci. 2015 Dec;56(13):7923-30
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5774825
Gene symbol:	Chd7
Gene name:	chromodomain helicase DNA binding protein 7

Antibody:	anti-CHD7
Detection system:	Secondary antibody

Results

Specimen 1A: embryonic day 9.5 (more

)

Structure	Level	Image	Note
TS15: eye surface ectoderm	Present	1A
TS15: optic vesicle	Present	1A	Expression is present in the neural ectoderm of the eye primordia.
TS15: eye mesenchyme	Absent	1A	No expression is seen in the periocular mesenchyme.

Specimen 1B: embryonic day 10.5 (more

)

Structure	Level	Image	Note
TS17: eye surface ectoderm	Present	1B
TS17: optic cup	Present	1B
TS17: optic stalk	Present	1B
TS17: eye mesenchyme	Absent	1B	No expression is seen in the periocular mesenchyme.

Specimen 1C: embryonic day 11.5 (more

)

Structure	Level	Image	Note
TS19: eye surface ectoderm	Present	1C
TS19: retina	Present	1C	Expression is seen in the inner and outer layers of the optic cup.
TS19: optic stalk	Present	1C
TS19: perioptic mesenchyme	Absent	1C	No expression is seen in the periocular mesenchyme.

Specimen 1D: embryonic day 12.5 (more

)

Structure	Level	Image	Note
TS20: cornea epithelium	Present	1D	Expression is present in the surface ectoderm of the eye.
TS20: retina	Present	1D	Expression is seen in the inner and outer layers of the optic cup.
TS20: optic stalk	Present	1D
TS20: perioptic mesenchyme	Absent	1D	No expression is seen in the periocular mesenchyme.

Specimen S1A: embryonic day 12.5 (more

)
Note: littermates were used for controls; their exact genotype was not detailed

Structure	Level	Pattern	Image	Note
TS20: retina	Present		S1A	Expression is present in ocular neural ectoderm.

Specimen S1B: embryonic day 12.5; Chd7^tm1.1Dmm/Chd7⁺, Tg(rx3-icre)1Mjam/0 (more

)
Note: Conditional mutant. these are neural ectoderm-specific heterozygotic embryos

Structure	Level	Pattern	Image	Note
TS20: retina	Present		S1B	Expression levels in the ocular neural ectoderm are reduced compared to those seen in wild type.

Specimen S1C: embryonic day 12.5; Chd7^tm1.1Dmm/Chd7^tm1.1Dmm, Tg(rx3-icre)1Mjam/0 (more

)
Note: Conditional mutant. these are neural ectoderm-specific knockout embryos

Structure	Level	Pattern	Image	Note
TS20: retina	Absent		S1C	Only occasional positive cells are seen in the ocular neural ectoderm.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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