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Gene Expression Data
Assay Details
Assay
Reference: J:171281 Nakagawa S, et al., Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice. J Cell Biol. 2011 Apr 4;193(1):31-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5770412
Gene symbol: Sfpq
Gene name: splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
Antibody: anti-PSF (clone B92)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 2C (2nd row): postnatal adult (more )
Note: Surface epithelial cells. Double labeled: pink - Sfpq; green - NEAT1 (short probe; assay MGI:5770403).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 2C merge (2nd row) , 2C PSF (2nd row) Expression concentrated in discrete large foci in surface epithelial cells.
Specimen 2C (3rd row): postnatal adult (more )
Note: Surface epithelial cells. Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 2C merge (3rd row) , 2C PSF (3rd row) Expression concentrated in discrete large foci in surface epithelial cells.
Specimen 2C (bottom row): postnatal adult (more )
Note: Surface epithelial cells. Double labeled: pink - Sfpq; green - Rbm14 (PSP2; assay MGI:5770410).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 2C merge (bottom row) , 2C PSF (bottom row) Expression concentrated in discrete large foci in surface epithelial cells.
Specimen 2D (2nd row): postnatal adult (more )
Note: Parietal cells. Double labeled: pink - Sfpq; green - NEAT1 (short probe; assay MGI:5770403).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 2D merge (2nd row) , 2D PSF (2nd row) Authors report uniform distribution of expression with some enrichment at putative transcription sites.
Specimen 2D (3rd row): postnatal adult (more )
Note: Parietal cells. Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 2D merge (3rd row) , 2D PSF (3rd row) Authors report uniform distribution of expression with some enrichment at putative transcription sites.
Specimen 2D (bottom row): postnatal adult (more )
Note: Parietal cells. Double labeled: pink - Sfpq; green - Rbm14 (PSF; assay MGI:5770410).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 2D merge (bottom row) , 2D PSF (bottom row) Authors report uniform distribution of expression with some enrichment at putative transcription sites.
Specimen 4D WT esophagus: postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: esophagus epithelium Present Regionally restricted 4D WT esophagus PSF , 4D WT esophagus (merge)
Specimen 4D KO esophagus: postnatal adult; Neat1tm1.1Shna/Neat1tm1.1Shna (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: esophagus epithelium Present Regionally restricted 4D KO esophagus PSF , 4D KO esophagus (merge) Authors report loss of punctate expression.
Specimen 4D WT forestomach: postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach proventricular region Present Regionally restricted 4D WT forestomach (merge) , 4D WT forestomach PSF
Specimen 4D KO forestomach: postnatal adult; Neat1tm1.1Shna/Neat1tm1.1Shna (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach proventricular region epithelium Present Regionally restricted 4D KO forestomach (merge) , 4D KO forestomach PSF Authors report loss of punctate expression.
Specimen 4D WT s. epithelium: postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 4D WT s. epithelium PSF , 4D WT s. epitehlium (merge)
Specimen 4D KO s. epithelium: postnatal adult; Neat1tm1.1Shna/Neat1tm1.1Shna (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 4D KO s. epithelium (merge) , 4D KO s. epitehlium PSF Authors report loss of punctate expression.
Specimen S2A (2nd row): postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (short probe; assay MGI:5770403).
Structure Level Pattern Image Note
TS28: stomach epithelium Present S2A PSF (2nd row) , S2A merge (2nd row) Authors state expression levels were highly variable between animals in certain tissues such as liver.
Specimen S2A (3rd row): postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach epithelium Present S2A merge (3rd row) , S2A PSF (3rd row) Authors state expression levels were highly variable between animals in certain tissues such as liver.
Specimen S2A (bottom row): postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - Rbm14 (PSF; assay MGI:5770410).
Structure Level Pattern Image Note
TS28: stomach epithelium Present S2A PSF (bottom row) , S2A merge (bottom row) Authors state expression levels were highly variable between animals in certain tissues such as liver.
Specimen S2B (2nd row): postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (short probe; assay MGI:5770403).
Structure Level Pattern Image Note
TS28: stomach epithelium Present S2B PSF (2nd row) , S2B merge (2nd row) Authors state expression levels were highly variable between animals in certain tissues such as liver.
Specimen S2B (3rd row): postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - NEAT1 (long probe; assay MGI:5770404).
Structure Level Pattern Image Note
TS28: stomach epithelium Present S2B PSF (3rd row) , S2B merge (3rd row) Authors state expression levels were highly variable between animals in certain tissues such as liver.
Specimen S2B (bottom row): postnatal adult (more )
Note: Double labeled: pink - Sfpq; green - Rbm14 (PSF; assay MGI:5770410).
Structure Level Pattern Image Note
TS28: stomach epithelium Present S2B PSF (bottom row) , S2B merge (bottom row) Authors state expression levels were highly variable between animals in certain tissues such as liver.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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