Pou2f2tm1c(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Pou2f2tm1c(EUCOMM)Hmgu |
Name: |
POU domain, class 2, transcription factor 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5767180 |
Synonyms: |
Pou2f2fl |
Gene: |
Pou2f2 Location: Chr7:24790111-24879292 bp, - strand Genetic Position: Chr7, 13.73 cM
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Alliance: |
Pou2f2tm1c(EUCOMM)Hmgu page
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IMPC: |
Pou2f2 gene page |
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Mutant Cell Line: |
HEPD0690_4_H11 |
Germline Transmission: |
Earliest citation of germline transmission:
J:231216
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 24796025 of Chromosome 7 upstream of the critical exons 8 through 11 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 8 through 11 at position 24797685. The critical exons 8 through 11 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:231216)
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Original: |
J:231216 Hodson DJ, et al., Regulation of normal B-cell differentiation and malignant B-cell survival by OCT2. Proc Natl Acad Sci U S A. 2016 Apr 5;113(14):E2039-46 |
All: |
2 reference(s) |
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