Foxa2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:160812 Ribes V, et al., Distinct Sonic Hedgehog signaling dynamics specify floor plate and ventral neuronal progenitors in the vertebrate neural tube. Genes Dev. 2010 Jun 1;24(11):1186-200
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5766916
Gene symbol:	Foxa2
Gene name:	forkhead box A2

Antibody:

anti-FoxA2

Results

1B ©

Specimen 1B: embryonic day 8.0 (more

)
Note: 7 somites. Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS12: neural tube floor plate	Present		1B
TS12: notochord	Present		1B
TS12: neural tube lateral wall	Present	Regionally restricted	1B	Expression is in the ventral midline.
TS12: future spinal cord neural plate	Present	Regionally restricted	1B	Expression is in the ventral midline of the anterior neural plate.

1C ©

Specimen 1C: embryonic day 8.0 (more

)
Note: 7 somites. Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS12: neural tube floor plate	Present		1C
TS12: notochord	Present		1C
TS12: neural tube lateral wall	Present	Regionally restricted	1C	Expression is in the ventral midline.
TS12: future spinal cord neural plate	Present	Regionally restricted	1C	Expression is in the ventral midline of the anterior neural plate.

1D ©

1D'' ©

Specimen 1D: embryonic day 8.5 (more

)
Note: 10 somites. Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS13: neural tube lateral wall	Present	Regionally restricted	1D'' , 1D	Expression is in the ventral midline.

1E ©

1E' ©

1E'' ©

Specimen 1E: embryonic day 9.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		1E'' , 1E , 1E'

1F ©

1F' ©

Specimen 1F: embryonic day 10.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		1F , 1F'

1I ©

Specimen 1I: embryonic day 9.5 (more

)
Note: Triple labeled: green - Foxa2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		1I

S1_A left ©

S1_A right ©

Specimen S1 A: embryonic day 9.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		S1_A left , S1_A right

S1_B left ©

S1_B right ©

Specimen S1 B: embryonic day 9.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		S1_B right , S1_B left
TS15: neural tube lateral wall	Present	Regionally restricted	S1_B right , S1_B left	Expression is in the ventral midline.

S1_C left ©

S1_C right ©

Specimen S1 C: embryonic day 9.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		S1_C left , S1_C right
TS15: neural tube lateral wall	Present	Regionally restricted	S1_C left , S1_C right	Expression is in the ventral midline.

S1_D left ©

S1_D right ©

Specimen S1 D: embryonic day 10.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1_D left , S1_D right

S1_E left ©

S1_E right ©

Specimen S1 E: embryonic day 10.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1_E right , S1_E left

S1_F left ©

S1_F right ©

Specimen S1 F: embryonic day 10.5 (more

)
Note: Triple labeled: red - Foxa2; green - Nkx2-2 (assay MGI:5766913); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1_F right , S1_F left

S1_I ©

Specimen S1 I: embryonic day 10.5; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^tm1Sor, Olig2^tm1(cre)Tmj/Olig2⁺ (more

)
Note: Conditional mutant. Lumbar level.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1_I

S1_I' ©

Specimen S1 I': embryonic day 10.5; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^tm1Sor, Olig2^tm1(cre)Tmj/Olig2⁺ (more

)
Note: Conditional mutant. Cervical level.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1_I'

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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