Probe:	Sno probe4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Weak		5A	Low level of expression in both embryonic and extraembryonic tissues.
TS11: extraembryonic component	Weak		5A	Low level of expression in both embryonic and extraembryonic tissues.

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present		5B	Authors report elevated expression in presomitic mesoderm
TS13: allantois	Present		5B	Elevated expression in allantois.

Structure	Level	Pattern	Image	Note
TS14: nervous system	Present		5C	Authors report increased expression in neural tissues.
TS14: future brain neural crest	Strong		5C	Strong expression in cranial neural crest cells.
TS14: 1st branchial arch	Present		5C	Expression in limb buds, mandibular arches and developing vasculature.
TS14: limb bud	Present		5C	Expression in limb buds, mandibular arches and developing vasculature.
TS14: vascular element	Present		5C	Expression in limb buds, mandibular arches and developing vasculature.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		5D	Expression in limb buds, mandibular arches and developing vasculature.
TS17: limb bud	Present		5D	Expression in limb buds, mandibular arches and developing vasculature.
TS17: vascular element	Present		5D	Expression in limb buds, mandibular arches and developing vasculature.
TS17: endocardial cushion	Present		5D
TS17: outflow tract	Present		5D
TS17: dorsal root ganglion	Present		5D

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		5E	Authors report expression first appeared above background in developing skeletal muscle. Expression in myotomes and abdominal muscle.
TS19: trunk musculature	Present		5E	Authors report expression first appeared above background in developing skeletal muscle. Expression in myotomes and abdominal muscle.
TS19: neural tube	Present	Regionally restricted	5E	In the neural tube, stronger expression in ependymal layer than in mantle layer.
TS19: hindlimb bud	Present	Regionally restricted	5E	Expression in distal progress zone of hindlimb buds.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Strong		5F	Highest levels of expression in central nervous system, skeletal muscle and limbs.
TS20: limb	Strong		5F	Highest levels of expression in central nervous system, skeletal muscle and limbs.
TS20: skeletal muscle	Strong		5F	Highest levels of expression in central nervous system, skeletal muscle and limbs.
TS20: heart	Present	Regionally restricted	5F	Expression in developing valves of the heart,
TS20: blood vessel	Present	Regionally restricted	5F	Expression in walls of blood vessels.

Structure	Level	Pattern	Image	Note
TS20: remnant of Rathke's pouch	Present
TS20: retina	Present
TS20: nasal epithelium	Present	Regionally restricted		Authors report expression in sinus epithelium.

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Strong		6A	Highest levels of expression in skeletal muscle, renal cortex, adrenal glands, gut, lung, and in the central nervous system
TS21: central nervous system	Strong		6A	Highest levels of expression in skeletal muscle, renal cortex, adrenal glands, gut, lung, and in the central nervous system
TS21: gut	Strong		6A	Highest levels of expression in skeletal muscle, renal cortex, adrenal glands, gut, lung, and in the central nervous system
TS21: lung	Strong		6A	Highest levels of expression in skeletal muscle, renal cortex, adrenal glands, gut, lung, and in the central nervous system
TS21: renal cortex	Strong		6A	Highest levels of expression in skeletal muscle, renal cortex, adrenal glands, gut, lung, and in the central nervous system
TS21: skeletal muscle	Strong		6A	Highest levels of expression in skeletal muscle, renal cortex, adrenal glands, gut, lung, and in the central nervous system
TS21: perichondrium	Present		6A	Expression in perichondrial zones.
TS21: genital swelling of genital tubercle	Present		6A	Expression in the genital eminence.
TS21: connective tissue	Present		6A	Expression in connective tissue of vertebral column,

Structure	Level	Pattern	Image	Note
TS22: forelimb muscle	Present		6C	Expression in skeletal muscles of forelimb and hindlimb.
TS22: hindlimb muscle	Present		6C	Expression in skeletal muscles of forelimb and hindlimb.
TS22: perichondrium	Present	Regionally restricted	6C	Expression in perichondrial cells of forelimb and hindlimb, especially at forming joints.
TS22: limb joint primordium	Present		6C	Expression in regions of developing joint in the limbs.

Structure	Level	Pattern	Image	Note
TS22: pharynx epithelium	Strong	Regionally restricted	6D	Strong expression in pharyngeal epithelium of the palate.
TS22: mandible	Present		6D
TS22: maxilla	Present		6D
TS22: brain	Present		6D

Structure	Level	Pattern	Image	Note
TS24: retina	Strong		6E
TS24: lens	Strong	Regionally restricted	6E	Prominent expression in the zone of proliferation of the lens.
TS24: vibrissa follicle	Present		6E

Structure	Level	Pattern	Image	Note
TS24: adipose tissue	Present	Regionally restricted		Expression in adipocytes.

Structure	Level	Pattern	Image	Note
TS27: skeletal muscle	Ambiguous			Authors report expression is decreased in skeletal muscle.
TS27: heart valve	Present			Expression remained elevated in cardiac valves and lung parenchyma.
TS27: lung parenchyma	Present			Expression remained elevated in cardiac valves and lung parenchyma.
TS27: brain	Present	Widespread

Structure	Level	Pattern	Image	Note
TS27: ovary	Present			Expression in ovaries and uterine epithelium.
TS27: uterus	Present	Regionally restricted		Expression in ovaries and uterine epithelium.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		6F	Predominant expression in neurons of the inner granule cell layer.
TS28: cerebellum molecular layer	Ambiguous		6F	Expression low or absent in molecular layer and white matter.
TS28: cerebellum white matter	Ambiguous		6F	Expression low or absent in molecular layer and white matter.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6G	Expression most evident in cerebrum and hippocampus.
TS28: telencephalon	Present		6G	Expression most evident in cerebrum and hippocampus.