Probe:	Pdgfc cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: branchial arch ectoderm	Strong		2A left
TS15: branchial arch membrane	Strong		2A left
TS15: branchial pouch	Strong		2A left
TS15: myotome	Strong		2A left
TS15: olfactory placode	Strong		2A left
TS15: oral region	Strong		2A left
TS15: limb mesenchyme	Strong	Regionally restricted	2A left	Expression is in the dorsal aspect in the presumptive myoblast.
TS15: foregut	Present	Regionally restricted	2A left	Expression is in the endoderm.
TS15: hindgut	Present	Regionally restricted	2A left	Expression is in the endoderm.
TS15: surface ectoderm	Weak	Regionally restricted	2A left	Expression is in the ventral surface ectoderm.
TS15: cardiac muscle tissue	Absent		2A left
TS15: central nervous system	Absent		2A left
TS15: cranial preganglion	Absent		2A left

Structure	Level	Pattern	Image	Note
TS15: branchial arch ectoderm	Strong		2A middle
TS15: branchial arch membrane	Strong		2A middle
TS15: branchial pouch	Strong		2A middle
TS15: myotome	Strong		2A middle
TS15: olfactory placode	Strong		2A middle
TS15: oral region	Strong		2A middle
TS15: limb mesenchyme	Strong	Regionally restricted	2A middle	Expression is in the dorsal aspect in the presumptive myoblast.
TS15: foregut	Present	Regionally restricted	2A middle	Expression is in the endoderm.
TS15: hindgut	Present	Regionally restricted	2A middle	Expression is in the endoderm.
TS15: surface ectoderm	Weak	Regionally restricted	2A middle	Expression is in the ventral surface ectoderm.
TS15: cardiac muscle tissue	Absent		2A middle
TS15: central nervous system	Absent		2A middle
TS15: cranial preganglion	Absent		2A middle

Structure	Level	Pattern	Image	Note
TS15: branchial arch ectoderm	Strong		2A right
TS15: branchial arch membrane	Strong		2A right
TS15: branchial pouch	Strong		2A right
TS15: myotome	Strong		2A right
TS15: olfactory placode	Strong		2A right
TS15: oral region	Strong		2A right
TS15: limb mesenchyme	Strong	Regionally restricted	2A right	Expression is in the dorsal aspect in the presumptive myoblast.
TS15: foregut	Present	Regionally restricted	2A right	Expression is in the endoderm.
TS15: hindgut	Present	Regionally restricted	2A right	Expression is in the endoderm.
TS15: surface ectoderm	Weak	Regionally restricted	2A right	Expression is in the ventral surface ectoderm.
TS15: cardiac muscle tissue	Absent		2A right
TS15: central nervous system	Absent		2A right
TS15: cranial preganglion	Absent		2A right

Structure	Level	Pattern	Image	Note
TS20: external auditory meatus	Strong		2B
TS20: fronto-nasal process epithelium	Strong		2B
TS20: nasal cavity epithelium	Strong		2B
TS20: oral region	Strong		2B
TS20: eye	Strong	Regionally restricted	2B	Expression is in the eyelid.
TS20: hair follicle	Strong	Regionally restricted	2B	Expression is in the outer cells of the follicle.
TS20: skeletal muscle	Present	Regionally restricted	2B	Expression is in myoblasts and myocytes of the facial and cervical muscles, the epaxial muscles of the trunk, the muscles in the limb bud and the intercostal muscles of the thorax.
TS20: intestine	Present	Regionally restricted	2B	Expression is in the intestinal loops that herniate through the umbilical hiatus.
TS20: skin	Weak	Regionally restricted	2B	Expression is in the ventral surface ectoderm.
TS20: central nervous system	Absent		2B
TS20: cranial ganglion	Absent		2B

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process epithelium	Present		3A
TS20: oral epithelium	Present		3A
TS20: olfactory epithelium	Present	Regionally restricted	3A	Expression is in neurogenic cells.

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process epithelium	Present		3B
TS20: oral epithelium	Present		3B
TS20: oral region	Present	Regionally restricted	3B	Expression is in the salivary gland epithelium.

Structure	Level	Pattern	Image	Note
TS20: surface ectoderm of genital tubercle	Present		3C
TS20: hindlimb mesenchyme	Present	Regionally restricted	3C	Expression is in the skeletal muscle.

Structure	Level	Pattern	Image	Note
TS21: hair follicle	Present	Regionally restricted	3D	Expression is in the outer root sheath cells.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present	Regionally restricted	3E	Expression is in the germinal cell layer.

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	3F	Expression is in the epithelium of the ducts.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted	3G	Expression is in the external granular layer.
TS21: forebrain	Absent		3G
TS21: midbrain	Absent		3G

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Regionally restricted	3H	Expression is in the proximal and distal segments of the bronchial tubules.

Structure	Level	Pattern	Image	Note
TS21: lobar bronchus epithelium	Present	Regionally restricted	3I	Expression is in the proximal and distal segments of the bronchial tubules.
TS21: lung epithelium	Present	Regionally restricted	3I	Expression is in the proximal and distal segments of the bronchial tubules.
TS21: lung mesenchyme	Present	Regionally restricted	3I	Expression is in the mesenchyme of the branching bronchial tubes.

Structure	Level	Pattern	Image	Note
TS20: small intestine	Present	Regionally restricted	3J	Expression is in the endodermal mucosal epithelium.
TS20: embryo mesenchyme	Present	Regionally restricted	3J	Expression is in the mesenchyme surrounding the gut.

Structure	Level	Pattern	Image	Note
TS21: small intestine	Present	Regionally restricted	3K	Expression is in the endodermal mucosal epithelium.
TS21: intestine muscularis	Present		3K

Structure	Level	Pattern	Image	Note
TS20: pretubular aggregate	Present	Regionally restricted	3L	Expression is in the mesenchyme and the epithelium.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present		3M
TS22: submandibular gland primordium epithelium	Present		3M

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present		3N
TS22: hip	Present	Regionally restricted	3N	Expression is in the perichondrium.

Structure	Level	Pattern	Image	Note
TS23: pretubular aggregate	Present	Regionally restricted	3O	Expression is in the mesenchyme and the epithelium.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present		3P
TS21: cardiac muscle tissue	Absent		3P

Structure	Level	Pattern	Image	Note
TS23: limb skeleton	Present	Regionally restricted	3Q	Expression is in osteoblasts of the trabecular bone.

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Present	Regionally restricted		Expression is in smooth muscle.
TS23: lung epithelium	Absent