Antibody:	anti-Rig-1
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1A	Expressed in the medial septum and the differentiating fields of the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1B	Expressed in the differentiating fields of the lateral and medial ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1C	Expressed in the preoptic area and the differentiating fields of the caudal ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	1D	Expressed in preplate.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	1E	Expressed in the medial septum.
TS20: ganglionic eminence	Strong		1E
TS20: lateral ganglionic eminence	Strong	Regionally restricted	1E	Expression is in the differentiating fields of the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	1F	Expressed in the preoptic area.
TS20: ganglionic eminence	Strong		1F
TS20: lateral ganglionic eminence	Strong		1F	Expression is in the differentiating fields of the lateral and medial ganglionic eminence.
TS20: medial ganglionic eminence	Strong		1F	Expression is in the differentiating fields of the lateral and medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	1G	Expressed in the preoptic area.
TS20: caudal ganglionic eminence	Strong	Regionally restricted	1G	Expression is in the differentiating fields of the caudal ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Strong		1H

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		1I

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		1J

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		1K

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex marginal layer	Present	Regionally restricted	2C , 2C'	Expressed within the lower half of the preplate/marginal zone and below a single layer of strongly labeled reelin immunopositive cells, a marker of Cajal--Retzius cells.
TS21: cortical preplate	Present	Regionally restricted	2C , 2C'	Expressed within the lower half of the preplate/marginal zone and below a single layer of strongly labeled reelin immunopositive cells, a marker of Cajal--Retzius cells.
TS21: cortical hem	Present	Regionally restricted	2C , 2C'	Colocalization with reelin was also more prominent within the deeper layers of the cortical hem.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	S1C	Expressed in the preplate layer, the lateral, medial and caudal ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present	Graded	S1F	In a decreasing mediolateral gradient.
TS20: lateral ganglionic eminence	Present	Regionally restricted	S1F	Robo1 and Robo3 showed similar patterns of expression both within the lateral and medial ganglionic eminences, and overlapped in regions where Robo1 protein was most robustly localized.
TS20: medial ganglionic eminence	Present	Regionally restricted	S1F	Robo1 and Robo3 showed similar patterns of expression both within the lateral and medial ganglionic eminences, and overlapped in regions where Robo1 protein was most robustly localized.

Structure	Level	Pattern	Image	Note
TS21: cortical hem	Strong	Regionally restricted	S1I	Expressed within the cortical hem, and this extended in a decreasing mediolateral gradient throughout the preplate/marginal zone.
TS21: cerebral cortex marginal layer	Strong	Graded	S1I	Expressed in a decreasing mediolateral gradient throughout the preplate/marginal zone.
TS21: cortical preplate	Strong	Graded	S1I	Expressed in a decreasing mediolateral gradient throughout the preplate/marginal zone.

Structure	Level	Pattern	Image	Note
TS19: forebrain ventricular layer	Present

Structure	Level	Pattern	Image	Note
TS20: caudal ganglionic eminence	Strong

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Present	Regionally restricted		Expressed in the medial septum.
TS26: hippocampus	Present
TS26: piriform cortex	Present			Expressed in the piriform and lateral cortices.