Probe:	Psap cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent
TS12: trophectoderm	Absent

Structure	Level	Pattern	Image	Note
TS28: decidua	Very strong	Regionally restricted		Expression is in the inner-zone decidual cells that surround the implanted embryo.

Structure	Level	Pattern	Image	Note
TS28: decidua capsularis	Strong		2a
TS16: embryo	Absent		2a
TS16: trophectoderm	Absent		2a

Structure	Level	Pattern	Image	Note
TS28: decidua basalis	Very strong		2b
TS23: spongiotrophoblast	Strong		2b
TS23: yolk sac	Present		2b
TS23: labyrinthine zone	Weak		2b
TS28: placenta	Weak		2b

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	2c	Expression is in the ependymal layer and the subependymal layer.
TS22: choroid plexus	Weak	Regionally restricted	2c	Expression is in the epithelium.
TS22: embryo	Weak	Diffuse	2c	Expression is in most embryonic tissues.

Structure	Level	Pattern	Image	Note
TS28: esophagus epithelium	Strong	Regionally restricted	2d	Expression is in the squamous mucosa.
TS28: trachea epithelium	Strong	Regionally restricted	2d	Expression is in the upper trachea.

Structure	Level	Pattern	Image	Note
TS28: cortical renal tubule	Strong	Regionally restricted	2e	Expression is in tubular epithelial cells.
TS28: medullary tubule	Weak	Regionally restricted	2e	Expression is in tubular epithelial cells.
TS28: glomerular tuft	Weak		2e

Structure	Level	Pattern	Image	Note
TS28: Harderian gland	Very strong	Regionally restricted	2f	Uniform expression in the cuboidal cells.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present
TS22: spinal cord	Weak

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Very strong		2g
TS28: choroid plexus	Very strong		2g
TS28: deep cerebellar nucleus	Very strong		2g
TS28: telencephalon	Very strong		2g
TS28: cerebellum	Very strong	Regionally restricted	2g	Expression is in neurons of the cerebellar stalk.
TS28: brain white matter	Weak		2g

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Weak	Regionally restricted	2h	Expression is in the lateral horn.
TS28: spinal cord ependymal layer	Moderate		2h
TS28: spinal cord white matter	Weak

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain ependyma		Strong
TS28: cerebellar cortex		Strong
TS28: cerebellum Purkinje cell layer		Strong
TS28: cerebral cortex		Strong
TS28: corpus luteum		Strong
TS28: cortical renal tubule		Strong
TS28: Sertoli cell	Sertoli cell	Strong
TS28: spinal cord		Strong	Regionally restricted		Expression is in lateral column neurons.
TS28: choroid plexus		Strong	Regionally restricted		Expression is in epithelial cells.
TS28: esophagus epithelium		Moderate
TS28: spinal cord ependymal layer		Moderate
TS28: mammary gland epithelium		Moderate			Lactating gland, weak expression in non-lactating glands.
TS28: lymph node medulla		Moderate	Regionally restricted		Expression is in macrophages.
TS28: thymus medullary core		Moderate	Regionally restricted		Expression is in macrophages.
TS28: liver		Moderate	Diffuse		Expression is in hepatocytes and is weaker in bile duct epithelium.
TS28: spleen		Present	Regionally restricted		Expression is in macrophages.
TS28: ciliary body		Present
TS28: retina		Present
TS28: pancreatic duct		Present	Regionally restricted		Expression is in the ductal epithelium.
TS28: lung alveolus		Present	Regionally restricted		Expression is in alveolar macrophages.
TS28: colon epithelium		Weak
TS28: duodenum epithelium		Weak
TS28: ileum epithelium		Weak
TS28: jejunum epithelium		Weak

Structure	Level	Pattern	Image	Note
TS28: jejunum mucosa	Moderate	Regionally restricted		Expression is in the lamina propria of the villi.
TS28: heart	Weak
TS28: lung	Weak
TS28: pelvic urothelial lining	Weak
TS28: salivary gland	Weak
TS28: thyroid gland	Weak
TS28: urothelium of bladder	Weak
TS28: urothelium of ureter	Weak
TS28: renal calyx	Weak	Regionally restricted		Expression is in epithelial cells.
TS28: bone marrow	Strong	Single cells		Expression is in mononuclear cells. Almost neutrophils were negative, and absent in megakaryocytes and in immature myeloid and erythroid cells.
TS28: stomach epithelium	Absent

Structure	Level	Pattern	Image	Note
TS26: cortical renal tubule	Present	Regionally restricted		Expression is in epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: epithelium of epididymis	Present
TS28: epithelium of seminal vesicle	Present
TS28: cauda epididymis	Weak
TS28: ductus deferens	Weak
TS28: prostate gland	Weak

Structure	Level	Pattern	Image	Note
TS28: endometrium glandular epithelium	Present
TS28: endometrium luminal epithelium	Present
TS28: epithelial layer of cervix uteri	Present
TS28: epithelial layer of oviduct	Present
TS28: epithelium of vagina	Present