Gene Expression Data
Assay Details
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Assay
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Reference:
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J:161846
Westfall JE, et al., Retinal degeneration and failure of photoreceptor outer segment formation in mice with targeted deletion of the Joubert syndrome gene, Ahi1. J Neurosci. 2010 Jun 30;30(26):8759-68
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5751132
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Gene symbol:
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Tubg1
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Gene name:
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tubulin, gamma 1
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Antibody:
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anti-g-Tubulin
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Detection system:
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Secondary antibody
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Assay notes:
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Fixative was 1% or 4 % paraformaldehyde. The specificity of the antibody used was not detailed; both family members have been annotated.
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Results
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Specimen
3B Ahi1+/+:
embryonic day 18.5
(more  )
Specimen
3B Ahi1+/+:
(close  )
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Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neural retina |
Present |
Regionally restricted |
3B Ahi1+/+
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Expression is in basal bodies in retinal photoreceptors.
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Specimen
3B Ahi1-/-:
(close )
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Genetic Background:
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involves: BALB/cJ * C57BL/6 * C57BL/6J
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Mutant Allele(s):
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Ahi1tm1Rujf/Ahi1tm1Rujf
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neural retina |
Present |
Regionally restricted |
3B Ahi1-/-
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Expression in this mutant is the same as in wild-type embryos.
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Analysis Tools