Probe:	Tmem163 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Frozen sections were postfixed with 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	1_Tmem163	Expression is in the subplate uniformly across the anterior--posterior expanse of the cortex.
TS28: cortical layer V	Weak		1_Tmem163
TS28: cortical layer VI	Absent		1_Tmem163

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2D	In this mutant all expressing subplate cells are shifted to the superplate. Absent at the white matter/gray matter boundary.
TS28: cerebral hemisphere white matter	Present	Single cells	2D

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2H	All expressing subplate cells are relocated to the middle of the cortical plate in this mutant.
TS28: cortical layer V	Absent		2H

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2L	Expression is in the subplate.

Structure	Level	Pattern	Image	Note
TS26: cortical subplate	Weak	Single cells	4_Tmem163 E18

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	4_Tmem163 P3	Expression is in the subplate.
TS27: cortical layer V	Trace		4_Tmem163 P3

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	4_Tmem163 P8	Expression is in the subplate.
TS28: cortical layer V	Present		4_Tmem163 P8

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	4_Tmem163 P21	Expression is in the subplate.
TS28: cortical layer V	Trace		4_Tmem163 P21

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Single cells	4_Tmem163 Adult	Expression is in the subplate.
TS28: cortical layer V	Trace		4_Tmem163 Adult