Antibody:	mpkcr-Ab
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: metanephric mesenchyme	Ambiguous			Authors report expression above background level but no clearly stained structures in kidneys prior to E13.5

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Regionally restricted	7A	Expression in the ureteric bud and collecting ducts.
TS23: ureteric tip	Weak		7A	Weakest expression at tip of bud.
TS23: nephrogenic interstitium	Absent		7A	No expression in uninduced mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Regionally restricted	7B	Expression in the ureteric bud and collecting ducts.
TS23: comma-shaped body	Absent		7B	No expression in in mesenchyme-derived structures: at early stages condensates, comma- or S-shaped bodies.
TS23: s-shaped body	Absent		7B	No expression in in mesenchyme-derived structures: at early stages condensates, comma- or S-shaped bodies.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Regionally restricted	7C	Expression in the ureteric bud and collecting ducts.
TS23: maturing glomerular tuft	Absent		7C	No expression in the developing or mature glomeruli.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Regionally restricted	7D	Expression in collecting ducts, more intense near the apical membrane.
TS23: mesenchyme of metanephros	Present	Regionally restricted	7D	No expression in differentiated stroma.
TS23: maturing glomerular tuft	Absent		7D	No expression in the developing or mature glomeruli.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Regionally restricted	7E	Expression more intense near the apical membrane.

Structure	Level	Pattern	Image	Note
TS26: inner medullary collecting duct	Strong	Regionally restricted	7F	High levels of expression in collecting ducts, in both papillary ducts in the medulla, and in straight collecting ducts of the periphery.
TS26: outer medullary collecting duct	Strong	Regionally restricted	7F	High levels of expression in collecting ducts, in both papillary ducts in the medulla, and in straight collecting ducts of the periphery.
TS26: inner renal medulla	Present	Regionally restricted	7F	Expression in renal papillae, slightly weaker than in the collecting ducts in the outer medulla.
TS26: ureter	Strong		7F
TS26: renal vasculature	Absent		7F	No expression in endothelial or smooth muscle cells in renal vessels.
TS26: metanephros	Present	Regionally restricted	7F	Spatial distribution of expression is maintained with no obvious decrease in the level of expression.

Structure	Level	Pattern	Image	Note
TS27: collecting duct	Present		7G
TS27: inner renal medulla	Present	Regionally restricted	7G	Expression in renal papillae, slightly weaker than in the collecting ducts in the outer medulla.
TS27: metanephros	Present	Regionally restricted	7G	Spatial distribution of expression is maintained with no obvious decrease in the level of expression.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		7H
TS28: inner renal medulla	Strong		7H	High levels of expression in renal papillae.
TS28: metanephros	Present	Regionally restricted	7H	Spatial distribution of expression is maintained with no obvious decrease in the level of expression.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		7I
TS28: inner renal medulla	Strong	Regionally restricted	7I	High levels of expression in renal papillae.
TS28: metanephros	Present	Regionally restricted	7I	Spatial distribution of expression is maintained with no obvious decrease in the level of expression.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Trace			Expression in collecting system decreased to a very low level, just above background levels.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Trace		7J	Expression in collecting ducts just above background levels.

Structure	Level	Pattern	Image	Note
TS27: hepatic duct	Strong		8A	High expression in epithelial cells of hepatic bile ducts.
TS27: liver	Weak	Regionally restricted	8A	Weak expression in hepatocytes and acinar cells.
TS27: portal vein	Absent		8A

Structure	Level	Pattern	Image	Note
TS27: pancreatic duct	Strong	Regionally restricted	8B	High expression in epithelial cells of pancreatic ducts.
TS27: pancreatic acinus	Weak		8B

Structure	Level	Pattern	Image	Note
TS27: intestinal villus	Present		8C

Structure	Level	Pattern	Image	Note
TS27: cardiac muscle tissue	Weak		8D

Structure	Level	Pattern	Image	Note
TS27: brain ependyma	Present		8E
TS27: choroid plexus	Present		8E

Structure	Level	Pattern	Image	Note
TS27: bronchiole	Present		8F

Structure	Level	Pattern	Image	Note
TS27: spleen	Absent