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Gene Expression Data
Assay Details
Assay
Reference: J:40260 Geng L, et al., Distribution and developmentally regulated expression of murine polycystin. Am J Physiol. 1997 Apr;272(4 Pt 2):F451-9
Assay type: Western blot
MGI Accession ID: MGI:5707460
Gene symbol: Pkd1
Gene name: polycystin 1, transient receptor potential channel interacting
Antibody: mpkcr-Ab
Detection system: Secondary antibody coupled to horseradish peroxidase
Results Image: 6
 Sample Information Bands Other Sample Information
Lane AgeStructure 400 kDa 70 Da 45 Da Genetic BackgroundMutant Allele(s)Sex
1 E10.5 (a) TS17: embryo Strong (b) Present Ambiguous Not Specified Not Specified
2 E11.5 (a) TS19: embryo Present Present Ambiguous Not Specified Not Specified
3 E12.5 (a) TS20: embryo Present Present Ambiguous Not Specified Not Specified
4 E13.5 (a) TS21: embryo Present (c) Ambiguous Ambiguous Not Specified Not Specified
5 E14.5 (a) TS22: embryo Weak Ambiguous Ambiguous Not Specified Not Specified
6 E14.5 (a) TS22: metanephros Strong (d) Ambiguous Ambiguous Not Specified Not Specified
7 E15.5 (a) TS23: embryo Weak Ambiguous Ambiguous Not Specified Not Specified
8 E15.5 (a) TS23: metanephros Strong (d) Ambiguous Ambiguous Not Specified Not Specified
9 E16.5 (a) TS24: embryo Weak Ambiguous (e) Ambiguous Not Specified Not Specified
10 E16.5 (a) TS24: metanephros Strong (d) Ambiguous Ambiguous Not Specified Not Specified
11 E17.5 (a) TS25: embryo Weak Ambiguous Ambiguous Not Specified Not Specified
12 E17.5 (a) TS25: metanephros Strong (d) Ambiguous Ambiguous Not Specified Not Specified
13 E18.0 (a) TS26: metanephros Strong (d) Present Ambiguous Not Specified Not Specified
14 P w 1 TS28: metanephros Strong (d) Present Ambiguous Not Specified Not Specified
15 P w 2 TS28: metanephros Weak (f) Weak (e) Present (g) Not Specified Not Specified
16 P w 3 TS28: metanephros Trace (h) Ambiguous (e) Present (g) Not Specified Not Specified
17 P w 4 TS28: metanephros Trace (i) Ambiguous (e) Present (g) Not Specified Not Specified
18 P w 5 TS28: metanephros Trace (i) Ambiguous (e) Present (g) Not Specified Not Specified
19 P w 6 TS28: metanephros Trace (i) Ambiguous (e) Ambiguous Not Specified Not Specified
20 P w 8 TS28: metanephros Trace (i) Ambiguous (e) Ambiguous Not Specified Not Specified
21 P adult TS28: metanephros Trace (i) Ambiguous (e) Ambiguous Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels highest in embryos at E10.5, and decrease thereafter.
(c) Expression starts to decrease in the total embryo at E13.5
(d) High level of expression in kidneys from E14 through 1 week after birth.
(e) Authors state the intensity of the 70 kDa band decreases in a similar manner to that of the 400 kDa band.
(f) Expression in the kidney begins to decrease at the 2nd postnatal week. Levels decrease at least 6-fold between the 1st and 2nd weeks.
(g) At 1 wk after birth, a new 45 kDa band appreas and increases in intensity.
(h) Expression decreased to trace level by the 3rd postnatal week.
(i) Authors state that a faint band could be seen in mice older than 3 weeks and in adult mice with prolonged exposure, indicating that a very low level of expression is maintained throughout life.

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory