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Gene Expression Data
Assay Details
Assay
Reference: J:54164 Turnley AM, et al., Cellular distribution and developmental expression of AMP-activated protein kinase isoforms in mouse central nervous system. J Neurochem. 1999 Apr;72(4):1707-16
Assay type: Northern blot
MGI Accession ID: MGI:5698413
Gene symbol: Prkaa2
Gene name: protein kinase, AMP-activated, alpha 2 catalytic subunit
Probe: AMPK a2 cDNA1
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Autoradiography
Results Image: 1A alpha2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex
E10 E10.0 (a) TS16: future brain Present Present 10 µg; total RNA CBA Not Specified
E14 E14.0 (a) TS22: brain Present Present 10 µg; total RNA CBA Not Specified
E16 E16.0 (a) TS24: brain Present Present 10 µg; total RNA CBA Not Specified
E19 E19.0 (a) TS26: brain Present Present 10 µg; total RNA CBA Not Specified
P2 P2 TS27: brain Present Present 10 µg; total RNA CBA Not Specified
P5 P5 TS28: brain Present Present 10 µg; total RNA CBA Not Specified
P8 P8 TS28: brain Present Ambiguous 10 µg; total RNA CBA Not Specified
P12 P12 TS28: brain Present Absent 10 µg; total RNA CBA Not Specified
P15 P15 TS28: brain Present Absent 10 µg; total RNA CBA Not Specified
P18 P18 TS28: brain Present Absent 10 µg; total RNA CBA Not Specified
P25 P25 TS28: brain Present Absent 10 µg; total RNA CBA Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Much smaller transcript detected between E10 and P8; at least 50% of total at E10; gradually decreased until virtually undetectable bby 8-12 days after birth.
(c) Dramatic increase in expression from E10-E14.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory