Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS17: blood	Strong	Regionally restricted	2A	High level of expression in large primitive erythroblasts circulating through the dorsal aorta.
TS17: embryo	Present	Single cells	2A	Authors report expression outside of blood vessels in the embryo proper. It was not clear whether these were erythroblasts or other cells migrating in the tissues.

Structure	Level	Pattern	Image	Note
TS20: blood	Weak	Regionally restricted	2B	Weak perinuclear expression in erythroblasts circulating to the heart.

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Regionally restricted	2C	Expression in definitive erythrocytes in the liver.

Structure	Level	Pattern	Image	Note
TS22: blood	Present	Regionally restricted	2D	Expression in only a few definitive erythrocytes in the heart.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Patchy	3A	Patchy expression in erythroid cells of different sizes, with expression more prevalent as cell size decreased.

Structure	Level	Pattern	Image	Note
TS28: spleen	Strong	Regionally restricted	3B	Expression only in mature erythrocytes.

Structure	Level	Pattern	Image	Note
TS28: blood	Present	Regionally restricted	3C	Expression in 10% of erythrocytes in peripheral blood.

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Present	Regionally restricted	4A	Expression restricted to tubular structures in the superficial region of renal cortex.

Structure	Level	Pattern	Image	Note
TS28: renal papilla	Absent		4B

Structure	Level	Pattern	Image	Note
TS28: renal medulla	Absent		4C

Structure	Level	Pattern	Image	Note
TS28: cortical renal tubule	Present	Regionally restricted	4D	Expression in tubular structures of the renal cortex that are short in length, likely to be distal convoluted tubules, connecting tubules and/or initial connecting tubules.
TS28: glomerular tuft	Absent		4D

Structure	Level	Pattern	Image	Note
TS28: aorta smooth muscle	Present		5A

Structure	Level	Pattern	Image	Note
TS28: inferior vena cava	Present	Regionally restricted	5B	Expression in smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	5C	Expression in smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS28: skin muscle	Present		5D	Expression in smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS12: somite	Absent

Structure	Level	Pattern	Image	Note
TS15: somite	Absent

Structure	Level	Pattern	Image	Note
TS17: somite	Weak	Patchy	6A	Patchy low level expression in rostral somites.

Structure	Level	Pattern	Image	Note
TS20: myotome	Present		6B	Higher expression in elongating myotome cells of rostral somites.

Structure	Level	Pattern	Image	Note
TS22: somite	Present		6C	Increased expression in rostral somites.

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Strong		6D	Strongest expression in adult skeletal muscle.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Absent		7A E10.5

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Absent		7A E11.5

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present		7A E12.5

Structure	Level	Pattern	Image	Note
TS21: lens	Present		7A E13.5

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	7C , 7B	Expression in lens fibers.

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Regionally restricted	7D	Expression only in cortical fiber cells, not is cells located toward the center of the lens.

Structure	Level	Pattern	Image	Note
TS27: retina	Weak	Scattered	8A	More expression in cells close to the pigment layer.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted		Expression confined to a single layer of cells.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted		Expression confined to a single layer of cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	8B	Authors report expression in cells oriented horizontally at the outer plexiform layer, with their cell bodies positioned in the inner nuclear layer.
TS28: retina outer plexiform layer	Present	Regionally restricted	8B	Authors report expression in cells oriented horizontally at the outer plexiform layer, with their cell bodies positioned in the inner nuclear layer.