Antibody:	anti-Bral2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: medial geniculate nucleus	Moderate		3K	More protein is detected than mRNA.

Structure	Level	Pattern	Image	Note
TS28: ventral posterior lateral thalamic nucleus	Moderate		5A
TS28: ventral posterior medial thalamic nucleus	Moderate		5A

Structure	Level	Pattern	Image	Note
TS28: superior olivary nucleus	Strong		5B

Structure	Level	Pattern	Image	Note
TS28: nucleus of trapezoid body	Strong		5C

Structure	Level	Pattern	Image	Note
TS28: lateral vestibular nucleus	Strong		5D

Structure	Level	Pattern	Image	Note
TS28: trigeminal nucleus	Present		5E	Expression in spinal trigeminal nucleus.

Structure	Level	Pattern	Image	Note
TS28: oculomotor nucleus	Moderate		5F

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Strong	Non-Uniform	5G	Strong staining in the ventral region, weak staining in the gray matter.

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Weak		5H	Expression in the dorso-medial part.
TS28: cortical layer V	Weak		5H	Expression in the dorso-medial part.

Structure	Level	Pattern	Image	Note
TS28: cochlear nucleus	Strong
TS28: red nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: subthalamic nucleus	Moderate

Structure	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus	Strong		7A

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present		7C

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	7E	Expression in this layer appears to be due to expression in the axon terminals of the stellate cells. There is also staining in the pinceau, where the terminals from basket cells converge and encapsulate each initial segment.
TS28: cerebellum granule cell layer	Present	Non-Uniform	7E	Expression in glomerular rosettes.

Structure	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus	Strong		7G	Expression in large neurons is pictured.

Structure	Level	Pattern	Image	Note
TS28: brainstem nucleus	Strong		9A
TS28: deep cerebellar nucleus	Strong		9A
TS28: gigantocellular reticular nucleus	Present	Non-Uniform	9A , 9C	There is perineuronal staining on the cell bodies and neurites.

Structure	Level	Pattern	Image	Note
TS28: gigantocellular reticular nucleus	Trace	Non-Uniform	9D , 9B	In the mutant staining is only visible at higher magnification. It is on the cell bodies.

Structure	Level	Pattern	Image	Note
TS28: pontine reticular nucleus	Present	Non-Uniform	9E	There is clear perinuclear staining.

Structure	Level	Pattern	Image	Note
TS28: pontine reticular nucleus	Trace	Non-Uniform	9F	In the mutant residues in the soma are weakly visible; unlike in wild type, there is no perineuronal staining seen.