Myl1 RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:34383 McGrew MJ, et al., Distinct gene expression patterns in skeletal and cardiac muscle are dependent on common regulatory sequences in the MLC1/3 locus. Mol Cell Biol. 1996 Aug;16(8):4524-34
Assay type:	RNase protection
MGI Accession ID:	MGI:5694629
Gene symbol:	Myl1
Gene name:	myosin, light polypeptide 1

Probe:	MLC3f cDNA1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	This blot was exposed overnight.

Results

Image: 3A

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
Soleus	P adult	TS28: soleus	Present	Present	2 µg; total RNA	C57BL/6		Not Specified
tRNA	Control
Atria	P adult	TS28: heart atrium	Present	Absent	2 µg; total RNA	C57BL/6		Not Specified
Ventricle	P adult	TS28: heart ventricle	Trace (c)	Absent	2 µg; total RNA	C57BL/6		Not Specified
Whole Heart	P adult	TS28: heart	Ambiguous	Ambiguous (d)	2 µg; total RNA	C57BL/6		Not Specified
Stomach	P adult	TS28: stomach	Absent	Absent	2 µg; total RNA	C57BL/6		Not Specified
Lung	P adult	TS28: lung	Absent	Absent	2 µg; total RNA	C57BL/6		Not Specified
Liver	P adult	TS28: liver	Absent	Absent	2 µg; total RNA	C57BL/6		Not Specified
Probe	Control

Notes:
(a) This band is the protected MLC3 transcript.
(b) This band is the protected MLC1 transcript.
(c) A band was seen with longer exposure times.
(d) It is possible there was a faint band in this lane.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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