Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174554
Gatz SA, et al., Requirement for DNA ligase IV during embryonic neuronal development. J Neurosci. 2011 Jul 6;31(27):10088-100
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5693888
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Gene symbol:
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Tubb3
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Gene name:
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tubulin, beta 3 class III
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Antibody:
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anti-beta-Tubulin III (T2200)
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Detection system:
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Secondary antibody
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Assay notes:
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Embryos were either snap frozen or fixed in 3% paraformaldehyde.
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Results
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Specimen
2a I:
embryonic day 14.5; Lig4tiny/ Lig4tiny
(more  )
Note: Double labeled with TUNEL (green).
Specimen
2a I:
(close  )
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Genetic Background:
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involves: C57BL/6JSfdAnu
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Mutant Allele(s):
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Lig4tiny/Lig4tiny
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Note:
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Double labeled with TUNEL (green).
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Specimen
2a II:
embryonic day 14.5; Lig4tiny/ Lig4tiny
(more )
Note: Double labeled with TUNEL (green).
Specimen
2a II:
(close )
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Genetic Background:
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involves: C57BL/6JSfdAnu
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Mutant Allele(s):
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Lig4tiny/Lig4tiny
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Note:
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Double labeled with TUNEL (green).
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Specimen
2b ctrl:
embryonic day 14.5
(more )
Note: The genetic background of the control specimen was either wild type or heterozygous. Double labeled with phosphoH3 (green).
Specimen
2b ctrl:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Note:
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The genetic background of the control specimen was either wild type or heterozygous. Double labeled with phosphoH3 (green).
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Specimen
2b -/-:
embryonic day 14.5; Lig4tiny/ Lig4tiny
(more )
Note: Double labeled with phosphoH3 (green).
Specimen
2b -/-:
(close )
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Genetic Background:
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involves: C57BL/6JSfdAnu
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Mutant Allele(s):
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Lig4tiny/Lig4tiny
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Note:
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Double labeled with phosphoH3 (green).
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Specimen
7c:
embryonic day 14.5
(more )
Note: Triple labeled with TUNEL (green) and BrdU (red).
Specimen
7c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Note:
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Triple labeled with TUNEL (green) and BrdU (red).
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