About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:174554 Gatz SA, et al., Requirement for DNA ligase IV during embryonic neuronal development. J Neurosci. 2011 Jul 6;31(27):10088-100
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5693888
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-beta-Tubulin III (T2200)
Detection system: Secondary antibody
Assay notes: Embryos were either snap frozen or fixed in 3% paraformaldehyde.
Results
Specimen 2a I: embryonic day 14.5; Lig4tiny/Lig4tiny (more )
Note: Double labeled with TUNEL (green).
Structure Level Pattern Image Note
TS22: forebrain Present 2a I TUNEL/Tuj1
Specimen 2a II: embryonic day 14.5; Lig4tiny/Lig4tiny (more )
Note: Double labeled with TUNEL (green).
Structure Level Pattern Image Note
TS22: forebrain Present 2a II TUNEL/Tuj1
Specimen 2b ctrl: embryonic day 14.5 (more )
Note: The genetic background of the control specimen was either wild type or heterozygous. Double labeled with phosphoH3 (green).
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present Regionally restricted 2b ctrl pH3/Tuj1 Expression is cells basal to the basal mitotic layer.
Specimen 2b -/-: embryonic day 14.5; Lig4tiny/Lig4tiny (more )
Note: Double labeled with phosphoH3 (green).
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present 2b Lig4Y288C pH3/Tuj1
Specimen 7c: embryonic day 14.5 (more )
Note: Triple labeled with TUNEL (green) and BrdU (red).
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present 7c Tuj1 , 7c TUNEL/BrdU/Tuj1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory