Probe:	Grg3 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: ectoplacental cone	Strong		4A
TS9: Reichert's membrane	Strong	Scattered	4A	May correspond to giant trophoblast cells that migrate from the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS11: chorion	Present		4B
TS11: embryo mesoderm	Present	Regionally restricted	4B	Expressed in the mesoderm migrating from the proximal region of the primitive streak.
TS11: embryo endoderm	Present	Regionally restricted	4B	A weaker signal anterior to the node, which may be the gut endodermal cells migrating forward from the node region of the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	4C	Expressed in the mesoderm migrating from the primitive streak region.
TS11: embryo endoderm	Present	Regionally restricted	4C	Expressed in the gut endoderm underlying the head folds.
TS11: allantois	Weak		4C	Also expressed in the cells migrating proximally into the extraembryonic region, destined to form the allantois.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	4D	Expressed in the neuroectoderm flanking the primitive streak.
TS12: lateral plate mesoderm	Present		4D
TS12: allantois	Weak		4D
TS12: somite	Present	Regionally restricted	4D	Expressed in the newly condensed somiteand at a slightly lower level in the condensed somites.
TS12: intermediate mesenchyme	Absent		4D
TS12: trunk unsegmented mesenchyme	Absent		4D

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		4E
TS12: future midbrain	Present		4E
TS12: gut endoderm	Present		4E
TS12: lateral plate mesoderm	Present		4E
TS12: somite	Present		4E
TS12: neural plate	Present	Widespread	4E	Throughout the neural plate.

Structure	Level	Pattern	Image	Note
TS16: neural plate	Present	Widespread	5A	Expressed throughout the neural plate.
TS16: mesenchyme derived from somatopleure	Present		5A

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	5B	Expressed in the most dorsal region and the ventricular zone.
TS16: dermomyotome	Present		5B
TS16: nephrogenic cord	Weak		5B

Structure	Level	Pattern	Image	Note
TS16: nasal pit	Present	Regionally restricted	5C	Expressed in the surface ectoderm in the region where the nasal placodes will form.
TS16: surface ectoderm	Present	Regionally restricted	5C	Expressed in the surface ectoderm in the region where the nasal placodes will form.
TS16: branchial arch	Present		5C
TS16: lens pit	Present		5C
TS16: future hindbrain	Present	Regionally restricted	5C	Expressed in the roof region.
TS16: future midbrain	Present	Regionally restricted	5C	Expressed in the roof region.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	5D	Expressed in the dorsal region.
TS16: somite	Present	Regionally restricted	5D	Expressed in the newly condensed somites.
TS16: dermomyotome	Present	Regionally restricted	5D	Expressed in earlier formed somites.

Structure	Level	Pattern	Image	Note
TS12: hindgut diverticulum endoderm	Present			Expressed in cells lining the hindgut pocket.
TS12: foregut diverticulum endoderm	Present			Expressed in cells lining the foregut pocket slightly later and at a lower level than in hindgut pocket.

Structure	Level	Pattern	Image	Note
TS16: dorsal root ganglion	Present