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Tg(RP23-370F21-GCaMP2)6Mik
Transgene Detail
Summary
Symbol: Tg(RP23-370F21-GCaMP2)6Mik
Name: transgene insertion 6, Michael I Kotlikoff
MGI ID: MGI:5693383
Synonyms: Acta2BAC-GCaMP2, B19-Acta2BAC-GCaMP2
Transgene: Tg(RP23-370F21-GCaMP2)6Mik  Location: unknown  
Alliance: Tg(RP23-370F21-GCaMP2)6Mik page
Transgene
origin
Strain of Origin:  (C57BL/6 x DBA/2)F2
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsThe ~184 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-370F21 was contains the entire actin alpha 2 locus (Acta2) as well as ~92 kbp of 5' flanking sequences (including the complete Fas locus) and ~68 kbp of 3' flanking sequences (including the complete Stambpl1 locus). Using homologous recombination/BAC recombineering, a GCaMP2-pA construct (GCaMP2 followed by an SV40 polyadenylation signal and flanking vector sequence) was inserted into the ATG start site of the BAC Acta2 gene (replacing the initiation codon of Acta2 in exon 2). No other loci on the BAC were altered. Mice from founder line 6 exhibit high sensor expression (GCaMP2 expression/EGFP fluorescence) in smooth muscle actin, including vessels. Transgene expression is consistent with endogenous Acta2.

The genetically encoded calcium indicator GCaMP2 is a calcium-sensing molecule composed of a 35 aa polyHis plasmid leader sequence (RSET; essential for thermal stability), a 13 residue peptide of chicken smooth muscle myosin light chain kinase (M13), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), and a rat calmodulin DNA fragment (CaM; aa 2-148). The cpEGFP mutations result in increased brightness (D180Y and V93I), thermal stability (V163A and S175G) and GFP dimerization prevention (A206K). (J:314125)

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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory