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Gene Expression Data
Assay Details
Assay
Reference: J:153562 Acampora D, et al., Selective inactivation of Otx2 mRNA isoforms reveals isoform-specific requirement for visceral endoderm anteriorization and head morphogenesis and highlights cell diversity in the visceral endoderm. Mech Dev. 2009 Oct;126(10):882-97
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5688750
Gene symbol: Otx2
Gene name: orthodenticle homeobox 2
Reporter: lacZ
Antibody: anti-B-galactosidase
Results
Specimen 9C: embryonic day 6.5; Otx2tm1Pas/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - Otx2 protein (assay MGI:5688184).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 9C The lacZ transcriptional control drove the expression of proximal isoforms and thereby, prevalently expressed in the visceral endoderm. Most, but not all Otx2-immunopositive cells were also beta-galactosidase-positive.
Specimen 9F: embryonic day 6.5; Otx2tm1Pas/Otx2tm9Asim (more )
Note: Double labeled: green - Otx2 reporter; red - Otx2 protein (assay MGI:5688184).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 9F The majority of Otx2-immunopositive visceral endoderm cells were also beta-galactosidase-positive.
Specimen 9I: embryonic day 6.5; Otx2tm1Pas/Otx2tm10Asim (more )
Note: Double labeled: green - Otx2 reporter; red - Otx2 protein (assay MGI:5688184).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 9I The majority of beta-galactosidase-positive visceral endoderm cells were Otx2-immunonegative.
Specimen 9L: embryonic day 6.5; Otx2tm1Pas/Otx2tm10Asim (more )
Note: Double labeled: green - Otx2 reporter; red - Otx2 protein (assay MGI:5688184).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 9L The majority of beta-galactosidase-positive visceral endoderm cells were Otx2-immunonegative.
Specimen S6A: embryonic day 7.5; Otx2tm1Pas/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - T (assay MGI:5688200).
Structure Level Pattern Image Note
TS11: primitive endoderm Present S6A Numerous beta-galactosidase-positive/Brachyury (T)-negative presumptive visceral endoderm cells were noted.
Specimen S6B: embryonic day 7.5; Otx2tm1Pas/Otx2tm1Pas (more )
Note: Double labeled: green - Otx2 reporter; red - T (assay MGI:5688200).
Structure Level Pattern Image Note
TS11: primitive endoderm Present S6B Numerous beta-galactosidase-positive/Brachyury (T)-negative presumptive visceral endoderm cells were noted.
Specimen S6C: embryonic day 7.5; Otx2tm1Pas/Otx2tm10Asim (more )
Note: Double labeled: green - Otx2 reporter; red - T (assay MGI:5688200).
Structure Level Pattern Image Note
TS11: primitive endoderm Present S6C Numerous beta-galactosidase-positive/Brachyury (T)-negative presumptive visceral endoderm cells were noted.
Specimen S6D: embryonic day 7.5; Otx2tm1Pas/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - Chrd (assay MGI:5688203).
Structure Level Pattern Image Note
TS11: primitive endoderm Present S6D Most of the beta-galactosidase-positive presumptive visceral endoderm cells were Chrd immunopositive.
Specimen S6E: embryonic day 7.5; Otx2tm1Pas/Otx2tm1Pas (more )
Note: DoDouble labeled: green - Otx2 reporter; red - Chrd (assay MGI:5688203).
Structure Level Pattern Image Note
TS11: primitive endoderm Present S6E Most of the beta-galactosidase-positive presumptive visceral endoderm cells were Chrd immunopositive.
Specimen S6F: embryonic day 7.5; Otx2tm1Pas/Otx2tm10Asim (more )
Note: Double labeled: green - Otx2 reporter; red - Chrd (assay MGI:5688203).
Structure Level Pattern Image Note
TS11: primitive endoderm Present S6F Most of the beta-galactosidase-positive presumptive visceral endoderm cells were Chrd immunopositive.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory