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Gene Expression Data
Assay Details
Assay
Reference: J:54649 Lambert de Rouvroit C, et al., Evolutionarily conserved, alternative splicing of reelin during brain development. Exp Neurol. 1999 Apr;156(2):229-38
Assay type: RT-PCR
MGI Accession ID: MGI:5646213
Gene symbol: Reln
Gene name: reelin
Probe: pF, pH
Probe preparation: labelled with P32
Visualized with: Phosphorimaging
Assay notes: The primer set used in this assay amplifies an alternative isoform. It has an alternative polyadenylation event resulting in the secretion of a truncated protein lacking the terminal, highly basic stretch.
Results Image: 4B--848
 Sample Information Bands Other Sample Information
Lane AgeStructure 848 bp Amount Genetic BackgroundMutant Allele(s)Sex
E12 + E12.5 (a) TS20: brain Present (b) 2 µg; total RNA BALB/c Not Specified
E12 - Control
E18 + E18.5 (a) TS26: brain Present (c) 2 µg; total RNA BALB/c Not Specified
E18 - Control
P0 + P0 TS27: brain Present (d) 2 µg; total RNA BALB/c Not Specified
P0 - Control
P5 + P5 TS28: brain Present (e) 2 µg; total RNA BALB/c Not Specified
P5 - Control
P10 + P10 TS28: brain Present (e) 2 µg; total RNA BALB/c Not Specified
P10 - Control
Ad + P adult TS28: brain Present (f) 2 µg; total RNA BALB/c Not Specified
Ad - Control
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) At this time this isoform represents 25 percent of the total reelin message.
(c) At this time this isoform represents 20 percent of the total reelin message.
(d) At this time this isoform represents 10 percent of the total reelin message.
(e) At this time this isoform represents 19 percent of the total reelin message.
(f) At this time this isoform represents 22 percent of the total reelin message.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory