Reln RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:54649 Lambert de Rouvroit C, et al., Evolutionarily conserved, alternative splicing of reelin during brain development. Exp Neurol. 1999 Apr;156(2):229-38
Assay type:	RT-PCR
MGI Accession ID:	MGI:5646213
Gene symbol:	Reln
Gene name:	reelin

Probe:	pF, pH
Probe preparation:	labelled with P32
Visualized with:	Phosphorimaging
Assay notes:	The primer set used in this assay amplifies an alternative isoform. It has an alternative polyadenylation event resulting in the secretion of a truncated protein lacking the terminal, highly basic stretch.

Results

Image: 4B--848

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	848 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
E12 +	E12.5 (a)	TS20: brain	Present (b)	2 µg; total RNA	BALB/c		Not Specified
E12 -	Control
E18 +	E18.5 (a)	TS26: brain	Present (c)	2 µg; total RNA	BALB/c		Not Specified
E18 -	Control
P0 +	P0	TS27: brain	Present (d)	2 µg; total RNA	BALB/c		Not Specified
P0 -	Control
P5 +	P5	TS28: brain	Present (e)	2 µg; total RNA	BALB/c		Not Specified
P5 -	Control
P10 +	P10	TS28: brain	Present (e)	2 µg; total RNA	BALB/c		Not Specified
P10 -	Control
Ad +	P adult	TS28: brain	Present (f)	2 µg; total RNA	BALB/c		Not Specified
Ad -	Control

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) At this time this isoform represents 25 percent of the total reelin message.
(c) At this time this isoform represents 20 percent of the total reelin message.
(d) At this time this isoform represents 10 percent of the total reelin message.
(e) At this time this isoform represents 19 percent of the total reelin message.
(f) At this time this isoform represents 22 percent of the total reelin message.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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