Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79898
Kochilas L, et al., The role of neural crest during cardiac development in a mouse model of DiGeorge syndrome. Dev Biol. 2002 Nov 1;251(1):157-66
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5645349
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Gene symbol:
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Tagln
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Gene name:
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transgelin
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
5A:
embryonic day 10.5; Taglntm1Msp/Tagln +
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Taglntm1Msp/Tagln+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Del(16Dgcr2-Hira)1Rak/+
Taglntm1Msp/Tagln+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 3rd arch artery |
Present |
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5B
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Expression is present in smooth muscle cells; no differences are observed between mutant and wild type.
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| TS17: 4th arch artery |
Absent |
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5B
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In the mutant no smooth muscle differentiation was seen in 6 of 10 right arches and 4 of 10 left arches.
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| TS17: 6th arch artery |
Absent |
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5B
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In the mutant no smooth muscle differentiation was seen in 5 of 10 right arches and 5 of 10 left arches.
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Specimen
5C:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Del(16Dgcr2-Hira)1Rak/+
Taglntm1Msp/Tagln+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 3rd arch artery |
Present |
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5C
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Expression is present in smooth muscle cells; no differences are observed between mutant and wild type.
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| TS17: 4th arch artery |
Absent |
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5C
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In the mutant no smooth muscle differentiation was seen in 6 of 10 right arches and 4 of 10 left arches.
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| TS17: 6th arch artery |
Trace |
Regionally restricted |
5C
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In the mutant smooth muscle differentiation was severely deficient; some was seen in 5 of 10 right arches and 5 of 10 left arches. It was located in the posterior aspect of the vessel.
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Specimen
not shown Ldgel/+:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Del(16Dgcr2-Hira)1Rak/+
Taglntm1Msp/Tagln+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 4th arch artery |
Trace |
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In the mutant smooth muscle differentiation was severely deficient; some was seen in 4 of 10 right arches and 6 of 10 left arches.
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Specimen
5D:
embryonic day 10.5; Taglntm1Msp/Tagln +
(more )
Note: coronal section
Specimen
5D:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Taglntm1Msp/Tagln+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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coronal section
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Specimen
5E:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Del(16Dgcr2-Hira)1Rak/+
Taglntm1Msp/Tagln+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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coronal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 3rd arch artery |
Present |
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5E
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Expression is present in smooth muscle cells; no differences are observed between mutant and wild type.
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| TS17: 4th arch artery |
Absent |
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5E
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In the mutant no smooth muscle differentiation was seen in 6 of 10 right arches and 4 of 10 left arches.
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Specimen
5F:
embryonic day 10.5; Taglntm1Msp/Tagln +
(more )
Note: Double labeled: blue - Tagln/SM22a; brown - actin (Acta2; assay MGI:5645339).
Specimen
5F:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Taglntm1Msp/Tagln+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tagln/SM22a; brown - actin (Acta2; assay MGI:5645339).
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Analysis Tools