Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO/Prnp-GCaMP3)600Cai
Name:	transgene insertion 600, Huaibin Cai
MGI ID:	MGI:5644521
Synonyms:	GC, tetO-GCaMP3
Transgene:	Tg(tetO/Prnp-GCaMP3)600Cai  Location: unknown
Alliance:	Tg(tetO/Prnp-GCaMP3)600Cai page

Strain of Origin:

C57BL/6J

Transgene Type:    Transgenic (Inducible, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: The transgenic construct contains the Tet-responsive element and mouse prion protein non-coding exons 1-2 upstream of a GCaMP3 cDNA sequence, followed by mouse prion protein non-coding exon 3 and polyadenylation signal. The GCaMP3 fusion gene cDNA sequence contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238] followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K and V93I mutations to increase dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to increase the fluorescence change for small calcium transients). (J:222395)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:222395 Sgobio C, et al., Optogenetic measurement of presynaptic calcium transients using conditional genetically encoded calcium indicator expression in dopaminergic neurons. PLoS One. 2014;9(10):e111749
All:	2 reference(s)