Antibody:	anti-Pins
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	These results were verified using two other antibodies to this protein: MGI:5640981 and MGI:5640983.

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present		5A	Expression is in the neuroblastic layer.
TS20: cornea epithelium	Present		5A

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present		5B	Expression is in the neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS22: lens epithelium	Present		5C	Expression is present in the anterior region of the lens (corresponding to the lens epithelium).

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present		5D	Expression is in the neuroblastic layer. It is stronger in the inner part of the layer.

Structure	Level	Pattern	Image	Note
TS26: neural retina	Present		5E	Expression is in the neuroblastic layer. It is stronger in the inner part of the layer.
TS26: cornea epithelium	Present		5E

Structure	Level	Pattern	Image	Note
TS26: neural retina	Present		5F	Expression is in the neuroblastic layer. It is stronger in the inner part of the layer. It is also observed in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS26: lens fiber	Present		5G	Expression is present in the posterior region of the lens (corresponding to the secondary lens fibers).

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		6B
TS27: neural retina	Present		6B	Expression is present in the neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		6C
TS28: neural retina	Present		6C	Expression is present in the neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		6D
TS28: retina outer nuclear layer	Present		6D
TS28: retina inner nuclear layer	Present	Non-Uniform	6D	Expression is more intense on either side of this layer than in its center.
TS28: retina inner plexiform layer	Present		6D
TS28: photoreceptor layer	Absent		6D
TS28: retina outer plexiform layer	Absent		6D

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		7B
TS28: retina inner nuclear layer	Present		7B
TS28: retina inner plexiform layer	Present		7B
TS28: retina outer nuclear layer	Present		7B
TS28: retina outer plexiform layer	Weak		7B

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		7C
TS28: retina inner plexiform layer	Present		7C
TS28: retina outer nuclear layer	Present		7C
TS28: retina inner nuclear layer	Present	Non-Uniform	7C	Expression is more intense on either side of this layer than in its center.
TS28: retina outer plexiform layer	Weak		7C
TS28: pigmented retinal epithelium	Present		7C
TS28: optic choroid	Present	Single cells	7C	Expression is present in melanocytes.
TS28: photoreceptor layer inner segment	Strong		7C

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Strong		8A
TS28: cornea epithelium	Strong		8A
TS28: cornea stroma	Strong		8A

Structure	Level	Pattern	Image	Note
TS28: ciliary epithelium	Present	Regionally restricted	8C	Expression is present in the nonpigmented cells but not the pigmented ones.

Structure	Level	Pattern	Image	Note
TS27: lens	Present	Diffuse	9A
TS27: hyaloid vascular plexus	Present		9A	Expression is present in the endothelial cells of the tunica vasculosa lentis, around red and white blood cells.

Structure	Level	Pattern	Image	Note
TS28: lens	Present	Diffuse	9B
TS28: hyaloid vascular plexus	Present		9B	Expression is present in the endothelial cells of the tunica vasculosa lentis, around red and white blood cells.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Strong		9C	Expression is present in the epithelial cells, transitional zone, and fiber cells.
TS28: lens fiber	Strong		9C	Expression is present in the epithelial cells, transitional zone, and fiber cells.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Strong		9D	Expression is present in the epithelial cells, transitional zone, and fiber cells.
TS28: lens fiber	Strong		9D	Expression is present in the epithelial cells, transitional zone, and fiber cells.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present		9E	Expression is present in the epithelial cells, transitional zone, and fiber cells.
TS28: lens fiber	Present		9E	Expression is present in the epithelial cells, transitional zone, and fiber cells.

Structure	Level	Pattern	Image	Note
TS28: vitreous humour	Present	Single cells	9G	Expression is present in hyalocytes.