Probe:	mPins-AS probe
Probe preparation:	Antisense labelled with digoxigenin DNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS27: neural retina	Strong		3A	Expression in the neuroblastic layer.
TS27: retina ganglion cell layer	Strong		3A

Structure	Level	Pattern	Image	Note
TS28: neural retina	Strong
TS28: retina ganglion cell layer	Strong

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer inner segment	Strong		3B
TS28: retina ganglion cell layer	Strong		3B
TS28: retina outer nuclear layer	Strong		3B
TS28: retina inner nuclear layer	Strong		3B , 3C	Labeling is more intense on either side of the inner nuclear layer (rather than in the middle).
TS28: retina inner plexiform layer	Absent		3C , 3B
TS28: retina outer plexiform layer	Absent		3C , 3B

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer inner segment	Strong		3D
TS28: retina ganglion cell layer	Strong		3D
TS28: retina inner nuclear layer	Strong		3D
TS28: retina outer nuclear layer	Strong		3D
TS28: retina inner plexiform layer	Absent		3D
TS28: retina outer plexiform layer	Absent		3D

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer inner segment	Strong		3E
TS28: retina ganglion cell layer	Strong		3E
TS28: retina inner nuclear layer	Strong		3E
TS28: retina outer nuclear layer	Strong		3E
TS28: retina inner plexiform layer	Absent		3E
TS28: retina outer plexiform layer	Absent		3E
TS28: optic nerve	Present		3E	Expression in cell bodies.
TS28: pigmented retinal epithelium	Strong		3F , 3E
TS28: optic choroid	Strong	Single cells	3F , 3E	Expression in choroidal melanocytes.
TS28: choroidal vasculature	Absent		3E , 3F	No expression in choroidal or uveal vascular endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Strong		4A
TS28: cornea epithelium	Strong		4A
TS28: cornea stroma	Strong	Single cells	4B , 4A	Expression is present in keratocytes.

Structure	Level	Pattern	Image	Note
TS28: ciliary epithelium	Present	Regionally restricted	4C	Expression is present in the nonpigmented cells but not the pigmented ones.

Structure	Level	Pattern	Image	Note
TS28: iris	Weak		4D

Structure	Level	Pattern	Image	Note
TS27: lens epithelium	Present		4E	Expression is present in the epithelial cells, transitional zone, and fiber cells.
TS27: lens fiber	Present		4E	Expression is present in the epithelial cells, transitional zone, and fiber cells.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present		4F	Expression is present in the epithelial cells, transitional zone, and fiber cells.
TS28: lens fiber	Present		4F	Expression is present in the epithelial cells, transitional zone, and fiber cells.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present		4G	Expression is present in the epithelial cells, transitional zone, and fiber cells.
TS28: lens fiber	Present		4G	Expression is present in the epithelial cells, transitional zone, and fiber cells.